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Multi-index analysis chip with pre-reaction function and its application method

An analytical chip and pre-reaction technology, applied in analytical materials, chemical instruments and methods, laboratory containers, etc., can solve the problems of inability to amplify detection and distribution of cells, so as to avoid cross-contamination, avoid contamination, The effect of reducing the possibility of cross-contamination

Active Publication Date: 2020-11-06
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Take the multi-indicator nucleic acid analysis of cells as an example, if there are only 5 cells in the sample, but there are 10 indicators to be analyzed, the samples are directly allocated to 10 chambers, and each chamber analyzes one indicator, there are many The cavity does not allocate cells, so the detection cannot be amplified

Method used

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  • Multi-index analysis chip with pre-reaction function and its application method
  • Multi-index analysis chip with pre-reaction function and its application method
  • Multi-index analysis chip with pre-reaction function and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] like figure 1 As shown, the multi-index analysis chip with pre-reaction function provided in this embodiment includes a substrate 1, and a reaction unit is arranged on one end surface of the substrate 1, and the reaction unit includes: a plurality of The reaction hole 101, the injection pipe 102 communicated with each reaction hole one by one, the distribution pipe 103 communicated with each injection pipe, the mixing chamber 105 communicated with the distribution pipe 103 through the siphon 104, the mixing chamber 105 communicated with the mixing chamber 105 through the connecting pipe 106 The pre-reaction chamber 107 and the reaction liquid chamber 108, and at least two sample injection holes 109 and at least one vent hole 110 communicated with the pre-reaction chamber 107 and the reaction liquid chamber 108 respectively.

[0056] The plurality of reaction holes 101 are located on the outermost side away from the centrifugal center, and are distributed at equal interv...

Embodiment 2

[0073] This embodiment is similar to the main structure of the chip described in Embodiment 1. The multi-index centrifugal analysis chip with pre-reaction function is as follows: Figure 4 As shown, there are a plurality of reaction holes 101, an injection pipe 102 communicating with each reaction hole one by one, a distribution pipe 103 connected with each injection pipe, a mixing chamber 105 communicating with the distribution pipe 103 through a siphon 104, and a mixing chamber 105. The pre-reaction chamber 107 and the reaction liquid chamber 108 communicated through the connecting pipe 106, and at least two sample injection holes 109 and at least one vent hole 110 respectively connected to the pre-reaction chamber and the reaction liquid chamber.

[0074] The plurality of reaction holes 101 are located on the outermost side away from the centrifugal center, and are distributed radially at equal angles with the centrifugal center as the center. The injection pipe 102 is loca...

Embodiment 3

[0081] Embodiment 1 and Embodiment 2 both show that a chip contains only one working unit. In fact, we can arrange multiple reaction units on the chip according to requirements. like Figure 5 As shown, the chip contains two groups of reaction units. The structure of the chip given in this example is similar to Example 1. Similarly, the structure in Example 2 can also be arranged into multiple repeating units. The chip structure, chip material, processing and use method of this embodiment are consistent with the embodiment, and will not be repeated here.

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Abstract

The invention discloses a multi-index analysis chip with a pre-reaction function and an application method thereof. The chip comprises a substrate, wherein the end surface of one side of the substratecomprises at least one reaction unit; each reaction unit comprises a plurality of reaction holes, injection tubes communicating with the reaction holes in a one-to-one correspondence manner, distribution tubes communicating with the injection tubes, a mixing chamber communicating with the distribution tubes via a siphon, at least one pre-reaction chamber and one reaction solution chamber communicating with the mixing chamber via connecting pipelines, and at least two sample introduction holes respectively communicating with the pre-reaction chamber and the reaction solution chamber; and the distribution tubes are composed of pipeline structures with different thickness and depth. The chip provided by the invention can be extensively applied to the field of biological detection or medicalexamination, e.g., biochemical analysis, immunoassay and nucleic acid amplification reactions.

Description

technical field [0001] The invention relates to a multi-indicator analysis chip with pre-reaction function and its application method. Background technique [0002] In recent years, microfluidic technology has developed rapidly, and its advantages in fluid manipulation and rapid biochemical reactions have enabled it to be applied in many fields. Biomedicine is an important field of its application. Microfluidic technology has been widely used in various applications such as cell culture, cell stimulation, cell analysis, nucleic acid extraction, nucleic acid amplification, biochemical detection, and immune detection. In the application of analyzing biochemical indicators, parallel analysis and detection of multiple indicators of samples is a common requirement. To realize the parallel analysis of multiple indicators of the same sample, it often involves the distribution process of samples and reagents, that is, the same liquid is distributed to multiple reaction wells for an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00B01L3/00
CPCB01L3/5027B01L3/50273B01L2200/10B01L2300/0861B01L2400/0409G01N35/00G01N2035/00495
Inventor 徐友春
Owner TSINGHUA UNIV
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