Sperm gradient centrifugation liquid and preparation method thereof

A gradient centrifugation and sperm technology, applied in the field of assisted reproduction, can solve the problems of limited pH buffer capacity, large pH fluctuation, and inability to reduce physical damage, and achieve strong buffer capacity and reduce sperm damage.

Active Publication Date: 2018-09-14
成都艾伟孚生物科技有限公司
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The buffer used in most sperm gradient centrifugation fluids is HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). The pH buffering capacity of a single buffer system is limited, and the pH fluctuation i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sperm gradient centrifugation liquid and preparation method thereof
  • Sperm gradient centrifugation liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Sperm Gradient Centrifugation Solution 3

[0058] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0059] 2) Add C component 10mmol / L and D component 4.10mmol / L, divide the solution into 2 equal parts, marked as solution 1 and solution 2;

[0060] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0061] 4) a1 and a2 were sterilized by filter membrane respectively.

Embodiment 2

[0062] Example 2 Sperm Gradient Centrifugation Solution 4

[0063] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0064] 2) Add C component 10mmol / L and D component 4.10mmol / L, add B component 30mg / L to divide the solution into 2 equal parts, marked as solution 1 and solution 2,

[0065] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0066] 4) a1 and a2 were sterilized by filter membrane respectively.

Embodiment 3

[0067] Example 3 Sperm Gradient Centrifugation Solution 5

[0068] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0069] 2) Add C component 10mmol / L and D component 4.10mmol / L, add B component 100mg / L to divide the solution into 2 equal parts, marked as solution 1 and solution 2,

[0070] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0071] 4) a1 and a2 were sterilized by filter membrane respectively.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of assisted reproduction, and particularly relates to sperm gradient centrifugation liquid and a preparation method thereof. According to the sperm gradient centrifugation liquid, pH (potential of hydrogen) of the sperm gradient centrifugation liquid is adjusted and controlled by HEPES+MOPS double-buffering systems, the pH is more stable, a key raw material lecithin is added into the sperm gradient centrifugation liquid, sperm activity is excited, and damage to sperms is repaired and reduced. Sperms selected by the sperm gradient centrifugation liquid are high in activity and higher in normal from rate, and full preparation is made for subsequent in-vitro fertilization.

Description

technical field [0001] The invention belongs to the field of assisted reproduction, and in particular relates to a sperm gradient centrifugal liquid and a preparation method thereof. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which human sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. A large number of people are unable to conceive naturally due to physical conditions, which requires in vitro fertilization-embryo transfer, commonly known as test-tube baby technology, for reproduction. According to a survey data, the infertility rate in my country has climbed from 2.5%-3% 20 years ago to 12.5%-15%. The number of newborns born in my country is about 16 million per year. Based on the infertility rate of 12.5%-15%, in theory, 2-2.4 million newborns cannot be born each year due to infertility. With the implementation of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/076
CPCC12N5/061C12N2509/10
Inventor 余裕炉米乐刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap