Detection method for influences of micro RNA-138-5p on human pancreatic cancer cell apoptosis under hypoxia

Abstract

The invention belongs to the technical field of medicinal preparations with organic and effective components, and discloses a detection method for influences of micro RNA-138-5p on human pancreatic cancer cell apoptosis under hypoxia. After up-regulation of miR-138-5p mimics, autophagy-associated protein of western blot detection experimental groups is inhibited; pancreas tumor cell autophagic vacuoles of confocal microscopy examination experimental groups are increased. The apoptotic cell percentage plus the necrotic cell percentage and differences between the groups have remarkable significance, and expression of apoptosis-associated protein of the western blot detection experimental groups is regulated up. MiR-138-5p and SIRT1 3'UTR regions are complementarily combined to inhibit reduction of SIRT1 expression. The miR-138-5p plays an important role in autophagy fluxes under hypoxia of pancreatic cancer cells, expression of SIRT1 is regulated through direct targeting to inhibit hypoxia-induced autophagy, and apoptosis of the panc-1 pancreatic cancer cells is promoted at the same time.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations containing organic active ingredients, and in particular relates to a method for detecting the effect of microRNA-138-5p on apoptosis of human pancreatic cancer cells under hypoxia. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Pancreatic cancer has a high degree of malignancy, the 5-year survival rate is less than 5%, and the prognosis of most patients is not very satisfactory. According to the 2015 Chinese cancer statistics, the death rate of pancreatic cancer ranks sixth among all cancers. The incidence of pancreatic cancer is gradually increasing every year, and it is likely to become the second most lethal disease in the near future. Therefore, it is of great significance to further elucidate the molecular mechanism of the occurrence and development of pancreatic cancer for its early diagnosis and develo...

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Problems solved by technology

[0003] In summary, the problems in the prior art are: At present, little is known about the relationship between miRNA and autophagy conditions in pancreatic cancer, the function of miRNA in pancreatic cancer is not completely clear, and its relationship with autophagy conditions is still unclear

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