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A liquid microbial fertilizer

A microbial fertilizer, liquid technology, applied in the direction of microorganisms, microorganisms, liquid fertilizers, etc., can solve the problems of environmental and food safety threats, and achieve the effect of improving quality and yield, improving utilization rate, and single active ingredient

Active Publication Date: 2022-02-01
NORTH CHINA PHARMA GROUP AINO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive use of chemical fertilizers poses a huge threat to China's environment and food safety

Method used

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  • A liquid microbial fertilizer
  • A liquid microbial fertilizer

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Comparison scheme
Effect test

Embodiment 1

[0059] A liquid microbial fertilizer, the active ingredient is methylotrophic bacillus, and the preparation steps are as follows:

[0060] First, the cryopreserved methylotrophic bacillus slant strains are activated, the activation is carried out in the seed tank, the bacterial strain is inoculated into the medium in the seed tank, and the inoculation amount is 0.1% of the volume of the seed tank culture medium, And adjust the fermentation condition in the seed tank to be tank pressure 0.05Mpa, air volume 2:10vvm, rotating speed be 250rpm, temperature be 35 ℃, pH value be 6.4, obtain activation liquid after cultivating 13h; The content is 1.5% of corn flour, 0.18% of bran, 0.25% of soybean cake powder, 0.06% of potassium dihydrogen phosphate dodecahydrate, 0.06% of anhydrous calcium carbonate, the rest is water, and the pH value is 6.4;

[0061] Then the activation solution is transferred to the fermenter, and the pressure of the fermenter is adjusted to 0.05Mpa, the air volum...

Embodiment 2

[0067]A liquid microbial fertilizer, the active ingredient is methylotrophic bacillus, and the preparation steps are as follows:

[0068] First the cryopreserved methylotrophic bacillus slant bacterial classification is activated, the activation is carried out in the seed tank, the bacterial strain is inoculated into the medium in the seed tank, and the inoculation amount is 0.3% of the volume of the seed tank culture medium, And adjust the fermentation conditions in the seed tank to be tank pressure 0.07Mpa, air volume 4:10vvm, rotating speed 220rpm, temperature 37°C, pH value 6.6, after cultivating for 11h to obtain the activation solution; each component of the seed tank culture medium and The content is 2.1% of corn flour, 0.12% of bran, 0.35% of soybean cake powder, 0.04% of potassium dihydrogen phosphate dodecahydrate, 0.08% of anhydrous calcium carbonate, the rest is water, and the pH value is 6.6;

[0069] Then the activation liquid is transferred to the fermenter, and...

Embodiment 3

[0075] A liquid microbial fertilizer, the active ingredient is methylotrophic bacillus, and the preparation steps are as follows:

[0076] First, the methylotrophic bacillus slant bacterial classification that is cryopreserved is activated, the activation is carried out in the seed tank, the bacterial strain is inoculated into the culture medium in the seed tank, and the inoculation amount is 0.2% of the volume of the seed tank culture medium, And adjust the fermentation condition in the seed tank to be tank pressure 0.06Mpa, wind volume be 3:10vvm, rotating speed be 235rpm, temperature be 36 ℃, pH value be 7, obtain activation liquid after cultivating 12h; Each component of seed tank culture medium and The content is 1.8% of corn flour, 0.15% of bran, 0.30% of soybean cake powder, 0.05% of potassium dihydrogen phosphate dodecahydrate, 0.07% of anhydrous calcium carbonate, the rest is water, and the pH value is 6.5;

[0077] Then the activation solution is transferred to the f...

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Abstract

The invention discloses a liquid microbial fertilizer and a preparation method thereof, belonging to the field of microbial application, including methylotrophic bacillus, which has been preserved in the China Typical Culture Collection Center on December 09, 2010 , the deposit number is CCTCC No: M2010343. The liquid microbial bacterial fertilizer of the present invention has few composition components, simple preparation method, single required bacterial species component, few miscellaneous bacteria, and long bacterial strain activity retention time; It has a strong control effect, can effectively improve the utilization rate of fertilizer and soil organic matter, regulate crop metabolism, promote the growth and development of crop root system, can significantly improve the quality and yield of crops, and has the advantages of non-toxic and harmless.

Description

technical field [0001] The invention relates to methylotrophic bacillus, in particular to a bacterial fertilizer, which belongs to the field of biological fertilizers. Background technique [0002] With the requirement of intensive agricultural production and sustainable development, crop diseases and insect pests have become the bottleneck restricting the sustainable development of modern agriculture. Various fungal diseases have caused a large number of crops and commercial crops to reduce or even fail to harvest, causing a lot of losses to the country and people's property. [0003] Botrytis cinerea is a common and difficult to control fungal disease of crops in open fields and protected areas. It is a low-temperature and high-humidity disease. The growth temperature of the pathogen is 20-30°C. period of high incidence of disease. Botrytis cinerea is caused by the infection of Botrytis cinerea, which is a fungal disease and can affect flowers, fruits, leaves and stems. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C05G3/60C05G5/20C12R1/07
CPCC12N1/20C05C11/00C05G3/00C05G5/27C12R2001/07C12N1/205C05F11/00C05F11/08
Inventor 赵学强胡晓敏李春田李欣苑丽蒲孟肖胡艳霞郎国桥
Owner NORTH CHINA PHARMA GROUP AINO
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