Cell viability detection device and method based on optical fiber digital coaxial holographic microscopy

Abstract

The invention provides a cell viability detection device and method based on optical fiber digital coaxial holographic microscopy and relates to the technical field of cell detection. The device comprises a laser light source emitting unit, an image collecting unit, an image acquisition unit, an objective table and a computer, wherein the upper-end plane of the objective table is provided with a transparent object-carrying window for placing cell sap, the laser source emitting unit is arranged at the lower end of the object-carrying window, the image acquisition unit is arranged at the upper end of the object-carrying window, and the output end of the image acquisition unit is connected with the input end of the computer. The device has the advantages that a cell sap hologram is determinedaccording to the fact that cells and an environment medium have different light refractive indexes and light passing the cells changes in phase as compared with light passing the environment medium,a Fourier transform method is used to reconstruct and invert cell section images, and arc tangent operation is used to obtain the true phase images of the cells; cell viability is determined accordingto the proportional relation between cell phase differences and refractive index differences; noninvasive and nondestructive quantitative imaging of biological cells is achieved.

Description

technical field [0001] The invention relates to the technical field of cell detection, in particular to a cell activity detection device and method based on an optical fiber digital coaxial holographic microscope. Background technique [0002] At present, the research on biological cells mainly includes the study of cell size distribution, shape characteristics, distribution of biological substances inside and outside cells, and cell dynamic behavior. However, most biological cells are colorless and transparent, and it is difficult to achieve clear imaging of cells by ordinary optical microscopy techniques. To overcome this difficulty, dyeing techniques are commonly used to change the color and brightness of cells, but this will cause damage to cells and affect cell performance. and dynamic behavior. Contents of the invention [0003] The main purpose of the present invention is to provide a cell activity detection device and method based on optical fiber digital coaxial ...

AI Technical Summary

Problems solved by technology

However, most biological cells are colorless and transparent, and it is difficult to achieve clear imaging of cells by ordinary optical microscopy techniques. To overcome this difficulty, dyeing techniques are commonly used to change the color and brightness of cells, but this will cause damage to cells and affect cell performance. and dynamic behavior

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