Unlock instant, AI-driven research and patent intelligence for your innovation.

Method of extraction and separation of chlorogenic acid from sweet potato leaves through ultrasonic-microwave-double water phase cooperation

A two-phase, ultrasonic technology, applied in chemical instruments and methods, separation/purification of carboxylic acid esters, preparation of carboxylic acid esters, etc., can solve the problems of long time and many steps, and achieve mild conditions, simple operation, and environmental protection. friendly effect

Active Publication Date: 2018-09-28
ZHEJIANG SHUREN UNIV
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many steps in this method, and the problem of long time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of two-phase aqueous system

[0040] Take 5g K 2 HPO 4 ·3H 2 O, add 50mL of 50% ethanol to prepare a two-phase aqueous extractant.

[0041] (2) Ultrasonic-microwave-water two-phase synergistic extraction

[0042] Take 25 mL of the two-phase aqueous system obtained in step (1), add 0.5 g of sweet potato leaf powder that has passed through a 60-mesh sieve, accurately weighed, the ratio of solid to liquid is 1:50 (g / mL), and the ultrasonic conditions are set to 200W, 30°C The treatment time was 8 minutes, and then the microwave treatment conditions were 480W and 1.5 minutes, and the ultrasonic-microwave-water two-phase synergistic extraction was carried out. After the extraction, the sweet potato chlorogenic acid extract with obvious phase separation was obtained by suction filtration, and the extraction rate was 4.26%. Separate the upper and lower phases, take the upper liquid, concentrate under reduced pressure (vacuum degree 0.1MPa, temperature 40°C)...

Embodiment 2

[0050] (1) Preparation of two-phase aqueous system

[0051] Take 5g K 2 HPO 4 ·3H 2 O, add 50mL50% ethanol to prepare a two-phase extractant;

[0052] (2) Ultrasonic-microwave-water two-phase synergistic extraction

[0053] Take 40 mL of the two-phase aqueous system obtained in step (1), add 0.5 g of sweet potato leaf powder that has passed through a 60-mesh sieve, and add 0.5 g of it, the ratio of solid to liquid is 1:80 (g / mL), and the ultrasonic conditions are set to 220W, 35°C The treatment time was 5 minutes, and then the microwave treatment conditions were 800W and 1 minute, and ultrasonic-microwave-water two-phase synergistic extraction was carried out. After the extraction, the sweet potato chlorogenic acid extract with obvious phase separation was suction-filtered, and the extraction rate was 4.32%. Separate the upper and lower phases, take the upper liquid, concentrate under reduced pressure (vacuum degree 0.1MPa, temperature 45°C), and vacuum dry (60°C, 3.5h) to...

Embodiment 3

[0055] (1) Preparation of two-phase aqueous system

[0056] Take 5g K 2 HPO 4 ·3H 2 O, add 50mL50% ethanol to prepare a two-phase extractant;

[0057] (2) Ultrasonic-microwave-water two-phase synergistic extraction

[0058] Take 50mL of the two-phase aqueous system obtained in step (1), add 0.5g of sweet potato leaf powder accurately weighed through a 60-mesh sieve, the material-to-liquid ratio is 1:100 (g / mL), and set the ultrasonic conditions to 180W, 25°C The treatment time was 10 minutes, and then the microwave treatment conditions were 560W and 1.5 minutes, and ultrasonic-microwave-water two-phase synergistic extraction was carried out. After the extraction, the sweet potato chlorogenic acid extract with obvious phase separation was suction-filtered, and the extraction rate was 4.42%. Separate the upper and lower phases, take the upper liquid, concentrate under reduced pressure (vacuum degree 0.1MPa, temperature 40°C), and vacuum dry (60°C, 3.5h) to obtain 0.0324g of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method of extraction and separation of chlorogenic acid from sweet potato leaves through ultrasonic-microwave-double water phase cooperation includes steps of: 1) adding inorganic salt to an ethanolwater solution being 35-65% in volume percentage, namely, performing phase separation to form a double water phase extraction agent; 2) adding the sweet potato leaf powder to the double water phase extraction agent and performing ultrasonic extraction at 25-35 DEG C for 5-10 min, ultrasonic wave power being 180-220 W; 3) performing extraction for 60-90 s under microwave power of 480-800 W; 4) performing suction filtration to obtain a filtrate and performing phase separation to obtain an upper-phase and a lower-phase extracts; 5) separating the upper and the lower phases to obtain an upper liquid and a lower liquid; 6) performing pressure reduced distillation concentration and vacuum drying to the upper liquid to prepare the final product, solid chlorogenic acid. The method has simple operations and integrated processes, is quick and high-effective, is environment-friendly, and has convenience in scale-up and continuous operation.

Description

(1) Technical field [0001] The invention relates to a method for extracting and separating sweet potato chlorogenic acid, in particular to a method for synergistic extraction and separation of sweet potato chlorogenic acid by ultrasonic wave-microwave and two-water phase. (2) Background technology [0002] Sweet potato (scientific name: Ipomoea batatas), also known as sweet potato, sweet potato, sweet potato, sweet potato, etc., is an annual herb of Convolvulaceae. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), sweet potatoes are grown in more than 100 countries all over the world. Currently, Asia and Africa are the main growing areas of sweet potatoes. Sweet potato is a major food crop in my country, and the planting area across the country is about 6.16×10 6 hm 2 , with a total output of 1.7×10 12 kg, the planting area and total output rank first in the world. It is the seventh largest food crop in the world and one o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/52C07C67/58C07C69/732
CPCC07C67/48C07C67/52C07C67/58C07C69/732Y02P20/10
Inventor 蒋新龙蒋益花
Owner ZHEJIANG SHUREN UNIV