The invention relates to a
microwave-assisted aqueous two-phase extraction method for separating
kudzu root total flavone, which comprises the following steps: 1) preparation of an aqueous two-phase
system: taking an inorganic salt, adding water to dissolve the inorganic salt, and adding an
organic solvent to obtain a aqueous two-phase extractant; and 2)
microwave-assisted aqueous two-phase extraction: adding
kudzu root
powder into the aqueous two-phase extractant obtained in the step 1), carrying out
microwave-assisted extraction, and carrying out vacuum
filtration to obtain an obvious phase-separated
kudzu root flavone extracting solution. Compared with the prior art, the method provided by the invention has the following advantages: (1) the method is simple to operate and does not need to wait for phase separation; 2) the process is integrated; 3) the extraction process is quick and efficient; 4) the extraction
solvent is low in
toxicity; 5) the interfacial tension is small, thereby being beneficial to
mass transfer between the two phases; 6) abundant impurities can be removed along with
solid matters, thereby being beneficial to purifying the sample; and 7) the extraction method does not have obvious scale-up effect, can easily implement technique amplification and
continuous operation, and can be directly connected with the subsequent purification procedure without special treatment.