Biflavonoid compound and application thereof
A technology of bisflavonoids and compounds, applied in the field of pharmacy, can solve the problem of enhancing the damage effect of carboplatin
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[0021] Example 1 The in vitro cell activity screening of the biflavones
[0022] 1.1 Test materials and instruments
[0023] 1.1.1 Test materials
[0024]A549 cells, HepG2 cells, Ovarian cells (BRCA gene deficient) Shanghai Cell Bank of Chinese Academy of Sciences; nude mice, Model Animal Center of Nanjing University; DMEM medium, Nanjing KGI Biotechnology Development Co., Ltd.; fetal bovine serum, ScienCell, USA; Anti (penicillin, streptomycin) Nanjing Kaiji Biotechnology Co., Ltd.; trypan blue, Canada BioBasic Co., Ltd.; thiazolyl tetrazolium (MTT), Nanjing Kaiji Biotechnology Development Co., Ltd.; Its structural analogs are isolated from Selaginella chinensis (S.Mollendorfii), and their structures are identified by NMR, MS and other spectral methods, with a purity of >95%.
[0025] 1.1.2 Test equipment:
[0026] Microplate reader, Tecan, Switzerland; Inverted fluorescence microscope, Nikon, Japan
[0027] 1.2 Test methods and results
[0028] 1.2.1 PARP-1 activity inh...
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