Microbial sample streak inoculation device and method

Abstract

The invention relates to the technical field of microbial sample processing, and in particular to a microbial sample streak inoculation device and a streak inoculation method. The streak inoculation device comprises a culture dish, a dish cover and a bacterium liquid injector, wherein the bacterium liquid injector comprises an injection pipe, an injection sleeve, a spring and a bacterium liquid storage bottle; a guide long hole, which is arranged in a width direction of the injection sleeve, is formed in the injection sleeve; a seal sleeve is arranged on the guide long hole; the injection sleeve penetrates the seal sleeve and the guide long hole and extends into the culture dish and the dish cover; the injection sleeve can slide along a length direction of the guide long hole; a streak guide groove is formed in the top surface of the inner wall of the dish cover; and a sliding block, which is matched with the streak guide groove, is arranged on the injection pipe. The microbial samplestreak inoculation device and the streak inoculation method provided by the invention, in comparison with the prior art, have the beneficial effects of being simple in operating steps of streak inoculation, high in efficiency of streak inoculation, low in error rate, capable of effectively preventing invasion of infectious microbes and good in effect of inoculating separated colones.

Description

technical field [0001] The invention relates to the technical field of microbial sample processing, in particular to a microbial sample streak inoculation device and method. Background technique [0002] Streak inoculation on a plate refers to diluting the mixed microorganisms or different cells in the same microbial population on the surface of the plate medium with an inoculation loop to obtain more independently distributed single cells, which grow and reproduce into single cells after culture. Colony, usually this single colony is regarded as a pure species of the microorganism to be isolated. The method for existing flat plate marking inoculation comprises: a, mark, on the bottom surface of the petri dish, indicate the name of the bacteria inoculated, the name of the inoculator, the date, etc. with a marker; Hold the inoculation loop, and put it in the flame to sterilize it; c. Take the strain, hold the test tube with the bacterial liquid in the left hand, use the palm...

AI Technical Summary

Problems solved by technology

The existing plate streak inoculation method has the following disadvantages: (1) In order to prevent as much bacteria as possible from entering the petri dish, it is necessary to burn and sterilize the inoculation loop and the strain test tube, and it is necessary to use the left thumb and index finger to control the streak inoculation. The dish cover, the remaining fingers of the left hand control the bottom of the dish, open the dish cover so that the opening angle is less than 30°, and close to the flame, the operation is very complicated, the efficiency is low, and it is easy to accidentally damage the petri dish, and bacteria are easy to enter when the dish cover is opened Petri dish; (2) It is necessary to take the strains many times, which is not only cumbersome to operate, but also easily leads to miscellaneous bacteria entering the petri dish; (3) The volume of the bacterial liquid taken out each time is not equal, and the bacteria cannot be effectively desalinated and separated after streaking. Affect the effect of inoculating isolated colonies

The streaking inoculation device for microbial samples does not need to open the cover of the culture plate during inoculation, can effectively prevent bacteria from entering, and does not need to use high-temperature disinfection. The operation is simple and the efficiency is high. However, the streaking inoculation device for microbial samples has the following disadvantages: (1) When the culture plate is placed vertically, the bacterial liquid will gather under the action of gravity to form drops after marking, which cannot effectively dilute the isolated bacteria and affect the effect of inoculation and isolation of colonies; (2) The bacterial liquid on the streaking inoculation pen There will be less and less streak inoculation, resulting in uneven bacterial liquid film formed by streak

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