Method for preventing PCR (Polymerase Chain Reaction) from forming aerosol contamination

Abstract

The invention discloses a method for preventing PCR (Polymerase Chain Reaction) from forming aerosol contamination. The invention provides the method for preventing aerosol contamination of a PCR product, comprising the following steps: 1) solidifying a liquid PCR reaction system into a solid state to obtain a solid-state PCR reaction system; 2) adding a template to the solid-state PCR reaction system for PCR amplification so as to prevent aerosol contamination of the PCR product. According to the invention, a low-melting-point agarose is added to the PCR reaction system; when the agarose becomes liquid during high-temperature PCR amplification reaction and melting curve analysis, the amplification and analysis of a target gene are not affected; when the temperature of a kit is returned toroom temperature after the completion of the analysis process, the agarose becomes solid again, and thereby a large amount of target gene fragments produced by amplification can be prevented from forming aerosol contamination. The agarose is easy to obtain, non-toxic and non-polluting, and no special treatment is needed after the reaction is completed.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for preventing PCR from forming aerosol pollution. Background technique [0002] Polymerase Chain Reaction (PCR) is an in vitro nucleic acid amplification technique developed in the 1980s. It has outstanding advantages such as specificity, sensitivity, high yield, rapidity, simplicity, good repeatability, and easy automation. In a test tube, the target gene or a certain DNA fragment to be studied can be amplified to 100,000 or even a million times within a few hours, so that the naked eye can directly observe and judge. PCR technology can amplify a sufficient amount of DNA from a hair, a drop of blood, or even a cell for analysis, research, detection and identification. PCR technology is a revolutionary initiative and milestone in the field of biotechnology, especially in the field of food safety testing, which shows its irreplaceable side. Although PCR technology is wide...

AI Technical Summary

Problems solved by technology

Although PCR technology is widely used, the biggest feature of its reaction is its large amplification ability and extremely high sensitivity, but extremely small amounts of pollution can also cause false positives, among which PCR amplification product pollution is the most common in PCR reactions. Main Most Common Contamination Problems

The copy amount of PCR product is far higher than the limit of several copies of PCR detection, so a very small amount of PCR product contamination can cause false positive

In addition, repeated heating during operation leads to aerosol pollution caused by opening the lid of the PCR reaction tube, etc., which is also a problem worthy of special attention.

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