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Acidic polyacrylamide electrophoresis separation method for Magnoliaceae plant seed prolamins

A technology of polyacrylamide and plant seeds, applied in the field of electrophoresis, which can solve the problems of low identification feasibility and high professional background requirements for researchers

Inactive Publication Date: 2018-09-28
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In summary, the problems in the prior art are: In practical applications, there are many cases of similar botanical characteristics among the varieties of magnolia plant seeds, which require high professional background for researchers, and the feasibility of identification through morphology is not high

Method used

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  • Acidic polyacrylamide electrophoresis separation method for Magnoliaceae plant seed prolamins
  • Acidic polyacrylamide electrophoresis separation method for Magnoliaceae plant seed prolamins

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Embodiment 1

[0031] (1) Extraction of gliadin in Magnoliaceae seeds

[0032] A. Accurately weigh 0.5 g of Magnolia guinan, Magnolia trichocarpa, and Magnolia magnolia among Magnoliaceae plants on an analytical balance, and add 2 mL of a sample that is dilute to 100 ml by 25% dichloroethanol+12% urea+1% mercaptoethanol The extract was ground in a mortar until homogenized, then transferred to a 5mL centrifuge tube, oscillated and mixed, centrifuged at 12000r / min for 20min at room temperature, and the supernatant was collected.

[0033] B. Take 1mL of the above supernatant in a 5mL test tube, add 1mL of 40% sucrose solution and a few drops of 0.5% methyl green, shake and mix well, and then centrifuge at 12000r / min for 10min at room temperature to collect the supernatant. Store at room temperature for later use.

[0034] (2) Preparation of electrophoresis reagents

[0035]A. Acrylamide stock solution: Weigh 29.2g of Acr (monomer acrylamide) and 0.8g of Bis (methylenebisacrylamide), add water...

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Abstract

The invention belongs to the technical field of electrophoresis, and discloses an acidic polyacrylamide electrophoresis separation method for Magnoliaceae plant seed prolamins. The acidic polyacrylamide electrophoresis separation method for Magnoliaceae plant seed prolamins comprises the following steps: extraction of prolamins in Magnoliaceae plant seeds, unidirectional rapid separation of the prolamins from the Magnoliaceae plant seeds, fixation, staining, and decolorizing. The method adopting acidic polyacrylamide electrophoresis to separate the Magnoliaceae plant seed prolamins is simple,is easy to perform, and provides a rapid and effective way for classifying and identifying seeds of different kinds of Magnoliaceae plants.

Description

technical field [0001] The invention belongs to the technical field of electrophoresis, and in particular relates to an acidic polyacrylamide electrophoresis separation method for magnolia plant seed gliadin. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Magnoliaceae plants are mostly tall evergreen trees, especially because of their gorgeous flower colors, pleasant fragrance, beautiful and polymorphic tree posture, and unique leaves and aggregate fruits. They are a kind of landscaping tree species with high ornamental value. However, the identification of Magnoliaceae seeds is a major difficulty encountered by gardeners when selecting species. How to quickly identify Magnoliaceae seeds is of great significance. Glamin is one of the components of plant seed storage protein. It cannot be dissolved in water or absolute ethanol, but it is soluble in 50%-90% ethanol, so people usually use ethanol as a solvent t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N27/447
CPCG01N1/30G01N27/447
Inventor 肖强王楠周秀梅洪健
Owner HUBEI UNIV FOR NATITIES
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