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Method for increasing nitrogen-use efficiency in plants

A plant-efficient technology, applied in botany equipment and methods, biochemical equipment and methods, plant peptides, etc., can solve problems such as agricultural losses

Active Publication Date: 2018-09-28
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Abiotic stress can negatively affect plant growth leading to severe agricultural losses

Method used

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  • Method for increasing nitrogen-use efficiency in plants
  • Method for increasing nitrogen-use efficiency in plants
  • Method for increasing nitrogen-use efficiency in plants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0215] 1.1. Materials and methods

[0216] Vector construction and rice transformation

[0217] We use Figure 10 The primers listed in amplified the OsNRT2.1 / OsNRT2.2 open reading frame (ORF) sequence from cDNA isolated from japonica rice Nipponbare (Oryza sativa L. ssp. Japonicacv. Nipponbare), in which the two genes were identical. We use Figure 11 The primers listed in amplify the OsNAR2.1 and ubiquitin promoters from the pOsNAR2.1(1698bp):GUS (Feng et al., 2011) and pUbi:OsPIN2 (Chen et al., 2012) constructs, respectively. The PCR product was cloned into pMD19-T vector (TaKaRa) and confirmed by restriction enzyme digestion and DNA sequencing. Such as Figure 15 The pUbi:OsNRT2.1 and pOsNAR2.1:OsNRT2.1 vectors were constructed as indicated. These constructs were introduced into Agrobacterium tumefaciens strain EHA105 by electroporation, which was then transformed into rice as previously described (Tang et al., 2012).

[0218] 1.1.1. Southern blot analysis

[0219] ...

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Abstract

Disclosed herein is a method of improving yield, growth and / or nitrogen use efficiency in plants comprising altering the expression profile of NRT. Also provided methods of making such plants, including nucleic acid constructs and genetically altered plants with the above traits.

Description

technical field [0001] The present invention relates to a method of increasing plant yield, growth and / or nitrogen use efficiency, said method comprising altering the expression profile of an NRT2 nucleic acid. The invention also relates to methods for producing such plants, including nucleic acid constructs and genetically altered plants having the above-mentioned traits. Background technique [0002] Nitrogen (N) nutrition affects all levels of plant function from metabolism to resource allocation, growth and development (Crawford, 1995; Scheible et al., 1997; Stitt, 1999; Scheible et al., 2004). The most abundant source of N for plant roots is nitrate (NO3-), which is present in naturally aerobic soils due to enhanced nitrification by applied organic N and fertilizer N. NO3-acts as a nutrient and as a signal to induce growth and changes in gene expression (Crawford and Glass et al., 1998; Wang et al., 2000; Zhang and Forde et al., 2000; Coruzzi and Bush et al., 2001; Cor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N5/14A01H1/00
CPCC07K14/415C12N15/8261C12N15/8271Y02A40/146C12N15/8222C12N15/8273
Inventor 范晓荣曹林
Owner NANJING AGRICULTURAL UNIVERSITY