A kind of method for the comprehensive utilization of whole biomass of tea
A technology of whole biomass and tea, applied in the field of comprehensive utilization of whole biomass of tea, can solve the problems of single low-value product, short industrial chain and low-end technology.
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Embodiment 1
[0033] According to the concentration of 0.25g / L, green tea is brewed with boiling water to prepare tea juice, and the tea juice and tea dregs are separated by filtration. Add white sugar at 12.5g / L to the tea juice, mix well, sterilize under high pressure steam at 115°C for 20 minutes, inoculate the Kombucha bacteria liquid prepared in advance, and cultivate it statically at 25°C until the pH value of the culture liquid reaches about 3. The fermentation can be stopped, and the obtained fermented liquid is a healthy tea drink; the obtained bacterial film is washed 3 times with distilled water, then immersed in 1% (w / w) NaOH solution, and bathed in 80°C for 120 minutes to remove the remaining bacteria and The culture medium was repeatedly washed with deionized water until neutral to obtain bacterial cellulose; tea leaves were crushed to 60 mesh, and potato juice was added at a ratio of 1:3 (w / v) to prepare a solid-state fermentation medium. After sterilization, According to 10%...
Embodiment 2
[0035] According to the concentration of 5g / L, brew black tea with boiling water to prepare tea juice, filter and separate the tea juice and tea dregs. Add brown sugar at 75g / L to the tea juice, mix well, sterilize at 115°C for 20 minutes, inoculate the Kombucha bacteria liquid prepared in advance, and culture at 30°C until the pH value of the culture solution reaches about 3. Stop the fermentation, and the obtained fermented liquid is a healthy tea drink; the obtained bacterial film is washed 3 times with distilled water, then immersed in a 1% (w / w) NaOH solution, and bathed in 80°C water for 120 minutes to remove the remaining bacteria and cultivate After repeated washing with deionized water until neutral, bacterial cellulose is obtained; tea leaves are crushed to 60 mesh, and distilled water is added at a ratio of 1:10 (w / v). After sterilization, laccase-producing bacteria are inoculated according to 10%. After culturing at 28° C. with shaking at 180 rpm for 7 days, the la...
Embodiment 3
[0037]Brew black tea with boiling water to prepare tea juice according to the concentration of 15g / L, filter and separate the tea juice and tea dregs. Add rock sugar at 125g / L to the tea juice, mix evenly, sterilize at 115°C for 20 minutes, inoculate the Kombucha bacteria liquid prepared in advance, and culture at 30°C until the pH value of the culture solution reaches about 3. Stop the fermentation, and the obtained fermented liquid is a healthy tea drink; the obtained bacterial film is washed 3 times with distilled water, then immersed in a 1% (w / w) NaOH solution, and bathed in 80°C water for 120 minutes to remove the remaining bacteria and cultivate After repeated washing with deionized water until neutral, bacterial cellulose is obtained; tea leaves are crushed to 60 mesh, and mixed with potato juice at a ratio of 1:5 (w / v) to prepare a solid-state fermentation medium. 10% was inoculated with laccase-producing strains, and after static culture at 30°C for 5 days, distilled...
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