Fe2+ ion for promoting proliferation of paenibacillus polymyxa and application method thereof
A technology of sporogenous polymyxa and its application method, which is applied in the fields of biopesticides and biomedicine, and can solve the problems of different effects of promoting proliferation, etc.
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[0006] Embodiment 1 a kind of Fe 2+ Ions promote the proliferation of Paenibacillus polymyxa and its application method
[0007] 1. Materials and methods
[0008] 1.1 Materials
[0009] The bacterial classification used in the present invention is endophytic Paenibacillus polymyxa of ginseng ( Paenibacillus polymyxa ), which was deposited on February 5, 2013 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 7250. Glucose, FeSO 4 ·7H 2 O (Beijing Chemical Industry). Purified water was from Hangzhou Wahaha Company; other reagents were of analytical grade.
[0010] 1.2 Instruments and equipment
[0011] CHA-S air bath reciprocating constant temperature shaking incubator; Tecan Infinite® 200 Pro multifunctional microplate reader.
[0012] 1.3 Method
[001...
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