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31results about How to "Shorten the delay period" patented technology

Bacillus amyloliquefaciens strain and application thereof

The invention discloses a Bacillus amyloliquefaciens strain and application thereof. The strain is Bacillus amyloliquefaciens ZTS-06, and the collection number is CGMCC No.11165. The invention also discloses a live strain preparation containing the strain and a preparation method thereof. The live strain preparation is prepared by the following steps: carrying out amplification culture on the Bacillus amyloliquefaciens ZTS-06, inoculating in a fermentation culture medium, culturing to obtain a fermentation liquid, adding propyl gallate, beta-cyclodextrin and soluble starch, and carrying out spray drying to obtain live strain raw powder; and adding abundant soluble starch, and uniformly mixing to obtain the live strain preparation. The strain has favorable degradation effects on phenylallyl propionic acid, para-hydroxybenzoic acid, cinnamic acid and the like, and can relieve succession cropping obstacles caused by phenolic acid and other toxic substances. The living strain preparation has the advantages of short fermentation period, high fermentation liquid bacterium concentration, low live strain loss in the spray drying process, and high stability in the storage process.
Owner:SHANDONG ZOETICLAND BIOLOGICAL TECH

Method for decomposing cellulose to produce hydrogen with cellulose clostridium

A method for using clostridium cellulosic to decompose cellulose and produce hydrogen relates to the method for decomposing the cellulose and producing the hydrogen. The method can solve the problems of long culture time, low utilization ratio of the cellulose and low hydrogen production rate of the prior method for utilizing the clostridium cellulosic H10 to decompose the cellulose and produce the hydrogen by fermentation. The method is as follows: 1. the clostridium cellulosic H10 is inoculated in a growth culture medium for culture; and 2. the clostridium cellulosic H10 after the culture is trans-inoculated in a hydrogen production culture medium for carrying out the anaerobic fermentation, thereby completing the decomposition of the cellulose and the hydrogen production by using the clostridium cellulosic. The delay period of the method is shortened by 20 percent to 30 percent, the utilization rate of the cellulose can be up to 65 percent to 75 percent and the hydrogen production rate achieves 1.9 plus or minus 0.1molH2 / mol hexose.
Owner:HARBIN INST OF TECH

Off-magnet patient scan positioning

ActiveCN101179989AReduce exposure to MRI scannersReduction of magnetic fields from radiation systems, etc.Patient positioning for diagnosticsDiagnostic recording/measuringRegion of interestMagnet
To position an imaging subject (16) in an imaging or therapy system (10), an imaging subject (16) is arranged on a linearly translatable tabletop (26) disposed on a movable trolley (30). A position of a region of interest (24) of the imaging subject along the linearly translatable tabletop is selected using an elongated position selector (40, 40', 40'') that is elongated in a translation direction (z) of the linearly translatable tabletop (26). After the selecting, the movable trolley is docked with the imaging or therapy system (10). After the docking, the tabletop is linearly translated to move the imaging subject to the imaging or therapy system, the amount of linear translating being based on the selected position of a region of interest along the linearly translatable tabletop.
Owner:KONINKLIJKE PHILIPS ELECTRONICS NV

Method for producing organic acid at high production rate through fermentation of intermittent backflow cells

The invention provides a method for producing organic acid at a high production rate through fermentation of intermittent backflow cells, which comprises the following steps of: performing filtration sterilization on a fresh culture medium, introducing into a fermentation tank, and inoculating high-density seed liquor into the fermentation tank; controlling organic acid fermentation production conditions of the used strain, when fermentation is performed until a small amount of sugar residue is in fermentation liquor, discharging partial or all fermentation liquor to a filtering device for processing, simultaneously supplementing a fresh germ-free culture medium into the fermentation tank, returning intercepted strain cell concentrated solution to the fermentation tank to serve as a production strain for later use, wherein the filtered liquor is used for extracting organic acid; and only when the production rate of the organic acid is obviously reduced, not returning the strain cell concentrated solution intercepted by the filtering device is not to the fermentation tank any more, and supplementing new high-density seed liquor and a fresh germ-free fermentation culture medium into the fermentation tank so as to perform a new round of production of organic acid at the high production rate through fermentation of the intermittent backflow cells. Through the method, high biomass and high production rate are always kept in the organic acid fermentation production process, and the method has high industrial application value.
Owner:HUAZHONG UNIV OF SCI & TECH

Lean burn engine and automobile

The invention belongs to the technical field of engines, and relates to a lean burn engine and an automobile. The lean burn engine comprises a water spraying system and an electric control unit, wherein the water spraying system comprises air flue water sprayers, pressure sensors and a water supply device; the air flue water sprayers are connected with the water supply device through water pipes;the pressure sensors are used for detecting the pressure values of the water pipes and sending the pressure values to the electric control unit; the air flue water sprayers are arranged on air inlet channels of air cylinders of the engine and spray water to the air inlet channels; the electric control unit is in signal connection with the air flue water sprayers and the pressure sensors; and whenthe engine is in different work conditions, the pressure values of the water pipes change, the pressure sensors send corresponding signals to the electric control unit according to the different pressure values of the water pipes, and the electric control unit controls the air flue water sprayers to adjust the water spraying amount. According to the lean burn engine, the electric control unit controls the air flue water sprayers to adjust the water spraying amount according to different engine work conditions (loads), and efficient clean combustion of the engine is achieved.
Owner:GUANGZHOU AUTOMOBILE GROUP CO LTD

Supplementary culture medium for shiitake mushroom fermentation and liquid submerged continuous culture method for shiitake mushrooms

The invention belongs to the technical field of liquid submerged fermentation of edible fungi and particularly relates to a supplementary culture medium for shiitake mushroom fermentation and a liquid submerged continuous culture method for shiitake mushrooms. The supplementary culture medium is prepared from, by weight, 4-6% of glucose, 0.5-1% of polypeptone, 0.5-1% of yeast extract powder, 0.1-0.3% of monopotassium phosphate, 0.05-0.15% of magnesium sulfate, 0.05-0.15% of calcium chloride, 0.3-0.5% of soybean oil, 0.01-0.03% of tween 80, 0.05-0.15% of acetic acid and 91-93% of water. The supplementary culture medium is comprehensive and balanced in nutrition and capable of effectively prolonging an exponential growth phase of the shiitake mushrooms, improving liquid submerged culture efficacy of the shiitake mushrooms and increasing biomass and lentinan yield when being used for liquid submerged continuous culture of the shiitake mushrooms.
Owner:SHANDONG LUKANG SHELILE PHARMA

High-content nicotinamide synthesis induced by new-feature nitrile hydratase

The invention discloses a method of producing nicotinamide through microorganism catalysis. According to a synergistic effect theory, a novel binary composite nitrile hydratase is disclosed, wherein the composite nitrile hydratase is produced through combined cultivation of bacillus pallidus and rhodococcus. The microorganism nitrile hydratase, which is characterized by being high in stereoselectivity, regioselectivity and being resistant to substrates and products in high concentration, is obtained by means of high substrate specificity and thermostability of the bacillus pallidus and a strong promoter effect of the rhodococcus. Meanwhile, by means of introduction of a co-solvent at a certain concentration to the reaction system, the reaction is accelerated. The method is simple and high-efficient, is environment-friendly, can reach not less than 99% in both purity and yield of the product and is suitable for large-scale industrial production.
Owner:ANHUI RUIBANG BIOLOGICAL SCI & TECH CO LTD

Saline-alkaline tolerant bacillus siamensis strain and production method and application of live bacteria preparation of saline-alkaline tolerant bacillus siamensis strain

The present invention discloses a saline-alkaline tolerant bacillus siamensis strain and a production method and an application of a live bacteria preparation of the saline-alkaline tolerant bacillussiamensis strain. The strain is isolated from the rhizosphere soil of rice and deposited in China General Microbiological Culture Collection Center on June 22, 2020, and has a preservation number of CGMCC No.20119. The bacillus siamensis PAPM-15 strain has strong saline-alkaline tolerance and strong ability to produce NH<3> and ACC deaminase, and the live bacteria preparation can effectively promote growth of rice, also has a relatively high resistance to rice bakanae disease caused by fusarium fujikuroi, and can be used to produce biological fertilizers, especially the biological fertilizersspecific for rice in saline-alkali soil.
Owner:SHANDONG ZOETICLAND BIOLOGICAL TECH +1

Saline-alkaline-tolerant Bacillus aerius and preparation method and application of living bacteria preparation of saline-alkaline-tolerant Bacillus aerius

The invention discloses saline-alkaline-tolerant Bacillus aerius and a preparation method and application of a living bacteria preparation of the saline-alkaline-tolerant Bacillus aerius. According tothe invention, the saline-alkaline tolerant Bacillus aerius PAPM-14 is separated from rhizosphere soil of corn. The strain is preserved in China General Microbiological Culture Collection Center on June 22, 2020, and the preservation number of the strain is CGMCC No. 20118. The strain has strong salt and alkali tolerance; the strain has strong IAA production, phosphate solubilization and potassium solubilization capabilities, has a strong antagonistic effect on pathogenic bacteria such as corn sheath blight disease, can be used for producing microbial fertilizers, especially microbial fertilizers special for saline-alkali soil corn, is used for biological prevention and control of crop diseases such as corn sheath blight disease, reduces the occurrence of diseases, and improves the yieldand quality.
Owner:德州八虎生物科技有限公司 +1

Salt-alkali-resistant streptomyces silaceus and application thereof

The invention discloses streptomyces silaceus PAPM 18 which is already collected at the China General Microbiological Culture Collection Center on July 4, 2018 under a biological collection number ofCGMCC NO. 16054. The strain can survive in saline-alkaline soil, has good degrading effect on plant autotoxins like allyl propionate, p-hydroxybenzoic acid and ferulic acid, can relieve successive cropping obstacle caused by the plant autotoxins and has effects of promoting growth of and increasing yield of cotton cultivated in saline-alkaline land. An active bacterial preparation can be obtainedby performing enlarged culture on the strain, inoculating the strain into a culture medium taking corn starch and corn paste as main nutrition sources, culturing at 35-37 DEG C for 72-96h, adding trehalose, molasses and biological fulvic acid powder into fermentation liquid and spray-drying. A preparation method of the active bacterial preparation is scientific, short in fermentation, high in fermentation liquid bacteria concentration, low in active bacteria loss in the process of spray-drying and high in stability of the active bacteria preparation in the process of preservation.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

A Salt-alkali-tolerant Pacific Bacillus and Its Live Bacterial Preparation and Application

The invention discloses a saline-alkali-tolerant pacific bacillus and its live bacterial preparation and application. A strain of Bacillus pacificus YJJK‑16 isolated from the rhizosphere soil of cabbage in saline-alkali land was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on July 26, 2019, with the preservation number CGMCC No.18300. Add propyl gallate, biochemical fulvic acid powder and cassonne to the fermented liquid of the bacterial strain and stir evenly to obtain live bacterial preparation. Bacillus pacificus YJJK‑16 has strong saline-alkali tolerance, has a high colonization rate in saline-alkali soil, and has a strong antagonistic effect on cabbage soft rot pathogens. It can be used to produce microbial fertilizers, especially microbial fertilizers for cabbage in saline-alkali soils. It is used in the biological control of cabbage soft rot, reducing the occurrence of diseases and improving yield and quality.
Owner:SHANDONG ZOETICLAND BIOLOGICAL TECH +2

Culture medium with composite trace elements and capable of promoting proliferation of paenibacillus polymyxa and application method of culture medium

The invention provides a culture medium with composite trace elements and capable of promoting proliferation of paenibacillus polymyxa and an application method of the culture medium, and provides appropriate concentration of the composite trace elements in the culture medium and a specific application method of the culture medium. The proliferation biomass of the paenibacillus polymyxa can be increased, the proliferation delaying phase of the paenibacillus polymyxa can be shortened, and the logarithmic phase of the proliferation can be advanced. The culture medium disclosed by the invention has important theoretical significance in high-efficiency culture of the paenibacillus polymyxa, and has high popularization value in the application of relevant biological microbial agents.
Owner:JILIN AGRICULTURAL UNIV

Application of a traditional Chinese medicinal agent in promoting the proliferation and/or secretion of lactic acid of Lactobacillus bulgaricus

The invention belongs to the technical field of cross application of food, health care products, medicine and the like, and particularly relates to application of a traditional Chinese medicine preparation to promoting multiplication of lactobacillus bulgaricus and / or secreting lactic acid. The traditional Chinese medicine preparation is prepared from the following components in parts by weight: 3-50 parts of radix et rhizoma rhei, 3-50 parts of moutan bark, 5-30 parts of peach seeds, 10-50 parts of of Chinese waxgourd seeds and 5-20 parts of mirabilite. The traditional Chinese medicine preparation has the advantages that the firstly provided traditional Chinese medicine preparation can integrally promote multiplication of lactobacillus in different periods, shortens the delay period of cultures, and prolongs rapid multiplication growth logarithmic phase, a high-level multiplication stable phase is maintained, the integral multiplication level and the number of the cultures are increased, enlargement and multiplication of cultures are facilitated, and the yield of metabolite L-lactic acid which is beneficial for physical health and generated by the cultures can be improved obviously. Therefore, the effects of the traditional Chinese medicine preparation on promoting multiplication of lactobacillus bulgaricus and secretion of lactic acid have good application prospects.
Owner:李超群

Mn2+ ion for promoting proliferation of paenibacillus polymyxa and application method thereof

The invention provides Mn2+ ion for promoting the proliferation of paenibacillus polymyxa and the application method thereof. An adopted culture medium is formed by adding Mn2+ into a PDB culture medium till the concentration is 50mg / L, and is equal to MnSO4.H2O with the final concentration of 153.75mg / L. The Mn2+ ion can improve the proliferation biomass of paenibacillus polymyxa, shorten the proliferation lag phase of paenibacillus polymyxa, and ensure that the log phase of proliferation is in advance. The Mn2+ ion has an important theoretical significance on high efficient culture of paenibacillus polymyxa, and has a wide promotion value for the application of relevant biological bacterium agents.
Owner:JILIN AGRICULTURAL UNIV

Method for promoting proliferation of paenibacillus polymyxa through Zn2+ ions and application method of paenibacillus polymyxa

The invention provides a method for promoting proliferation of paenibacillus polymyxa through Zn2+ ions and an application method of paenibacillus polymyxa. According to the method, a culture medium is prepared in the mode that Zn2+ is added to a PDB culture medium until the final concentration of the PDB culture medium is 100 mg / L, and the culture medium is equivalent to ZnSO4.7H2O with the concentration of 439.75 mg / L. According to the method, the biomass of proliferation of paenibacillus polymyxa can be increased, the delay period of proliferation of paenibacillus polymyxa is shortened, andthe logarithmic phase of proliferation is shortened. The method has important theoretical significance on efficient culture of paenibacillus polymyxa and has high popularization value on applicationof related antibiological inoculants.
Owner:JILIN AGRICULTURAL UNIV

Sedimentation-reverse perfusion-flow coupling method for preparing DHA-rich oil and its special cell sedimentation tank

ActiveCN104195187BHelps relieve the problem of reduced activityRelieves the problem of reduced activityBioreactor/fermenter combinationsBiological substance pretreatmentsRefluxOil and grease
The invention relates to a method for preparing grease rich in DHA and special equipment therefor. The method comprises the following steps: in an aeration fermentation tank containing a fermentation culture medium, cultivating schizochytrium CGMCCNo.7693 in a mechanical stirring manner; and extracting and recovering the grease rich in DHA from schizochytrium cells, wherein the culture process comprises continuous fed-batch cultivation of a medium, sedimentation-adverse perfusion of cells, and periodical discharge of fermentation liquor; the special cell sedimentation tank comprises a primary sedimentation tank, a secondary sedimentation tank, seal covers, openings and outlets; the primary sedimentation tank and the secondary sedimentation tank are arranged at an interval and are of conical shapes in appearance; the seal covers are arranged on the primary sedimentation tank and the secondary sedimentation tank; the openings are formed in the seal covers; the outlets are formed in the bottoms of the primary sedimentation tank and the secondary sedimentation tank; a liquid inlet pipe is arranged on the primary sedimentation tank; a supernate discharge pipe is arranged at the upper part of the secondary sedimentation tank; the primary sedimentation tank and the secondary sedimentation tank are connected through a communicating pipe; and the outlets are connected with a fermentation tank through concentrated cell reflux pumps and pipes. According to the method and the special cell sedimentation tank, the problems in a micro algae continuous cultivation technique that cells are difficultly intercepted and cell loss is serious are solved, the special cell sedimentation tank has the advantages of reduction of manual operation, easiness in automatic production, short production cycle and the like.
Owner:RUNKE BIOENG FUJIAN

Method for producing organic acid at high production rate through fermentation of intermittent backflow cells

The invention provides a method for producing organic acid at a high production rate through fermentation of intermittent backflow cells, which comprises the following steps of: performing filtration sterilization on a fresh culture medium, introducing into a fermentation tank, and inoculating high-density seed liquor into the fermentation tank; controlling organic acid fermentation production conditions of the used strain, when fermentation is performed until a small amount of sugar residue is in fermentation liquor, discharging partial or all fermentation liquor to a filtering device for processing, simultaneously supplementing a fresh germ-free culture medium into the fermentation tank, returning intercepted strain cell concentrated solution to the fermentation tank to serve as a production strain for later use, wherein the filtered liquor is used for extracting organic acid; and only when the production rate of the organic acid is obviously reduced, not returning the strain cell concentrated solution intercepted by the filtering device is not to the fermentation tank any more, and supplementing new high-density seed liquor and a fresh germ-free fermentation culture medium into the fermentation tank so as to perform a new round of production of organic acid at the high production rate through fermentation of the intermittent backflow cells. Through the method, high biomass and high production rate are always kept in the organic acid fermentation production process, and the method has high industrial application value.
Owner:HUAZHONG UNIV OF SCI & TECH

Production method and application of a salt-alkali-tolerant bacillus siamese and its live bacterial preparation

The invention discloses a production method and application of a saline-alkali-resistant bacillus siamese and a live bacterial preparation thereof. The strain was isolated from the rice rhizosphere soil, and was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on June 22, 2020, with the preservation number CGMCC No.20119. Bacillus siamese PAPM‑15 strain has strong saline-alkali tolerance and produces NH 3 and ACC deaminase, its live bacteria preparation can effectively promote the growth of rice, and has a high control effect on rice bakanae disease caused by Fusarium vine nigricans, and can be used to produce biological fertilizers, especially rice special organisms in saline-alkali land fertilizer.
Owner:SHANDONG ZOETICLAND BIOLOGICAL TECH +1

Pond substrate modifier as well as preparation method and application thereof

The invention relates to a pond substrate modifier, which is prepared from the following ingredients in parts by mass: 10 to 15 parts of zeolite powder, 30 to 55 parts of sodium humate, 10 to 20 parts of compound bacterium powder, 5 to 10 parts of compound enzyme, 10 to 20 parts of hydroxypropyl starch, 5 to 10 parts of protective agents and 5 to 15 parts of stabilizing agents, wherein the compound bacterial powder comprises bacillus subtilis bacterial powder, bacillus licheniformis bacterial powder, paracoccus denitrificans bacterial powder and thiobacillus denitrificans bacterial powder; and the pond substrate modifier has a double-layer structure. The invention further relates to a preparation method of the pond sediment modifier and application of the pond sediment modifier in the field of aquaculture. The pond sediment modifier can decompose organic matters at the bottom of a pond, reduce the content of ammonia nitrogen, nitrite and sulfide, and improve the transparency of a water body at the same time.
Owner:江门市澳保生物科技有限公司

Culture medium for liquid fermentation of edible and medicinal fungi

The invention relates to a culture medium for the liquid fermentation of edible and medicinal fungi. The culture medium comprises KH2PO4, MgSO4, sucrose, peptone and corn residue, wherein the corn residue is replaced by one of rice bran, sorghum flour, wheat grains and wheat bran. The obtained culture medium has low cost and high economic value.
Owner:武侯区巅峰机电科技研发中心

A strain of saline-alkali-tolerant Streptomyces ochreus and its application

The present invention discloses a strain of Streptomyces silaceus PAPM18, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on July 4, 2018, and its biological preservation number is: CGMCC NO.16054 . The strain can survive in saline-alkali soil, and has a good degradation effect on plant autotoxic substances such as phenylacrylic acid, p-hydroxybenzoic acid, and ferulic acid, and can reduce continuous cropping obstacles caused by them. Promote growth and increase yield. After expanding the culture, the strain was inoculated in a medium with cornstarch and corn steep liquor as the main nutrient source, cultured at 35-37°C for 72-96h, and then trehalose, molasses and biological fulvic acid powder were added to the fermentation broth, and then The live bacteria preparation can be obtained by spray drying. The preparation method of the microbial preparation is scientific, the fermentation period is short, the fermentation liquid bacterial concentration is high, the loss of live bacteria is small in the spray drying process, and the stability of the live bacteria preparation is high in the preservation process.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Application of traditional Chinese medicine preparation to promoting multiplication of lactobacillus bulgaricus and/or secreting lactic acid

The invention belongs to the technical field of cross application of food, health care products, medicine and the like, and particularly relates to application of a traditional Chinese medicine preparation to promoting multiplication of lactobacillus bulgaricus and / or secreting lactic acid. The traditional Chinese medicine preparation is prepared from the following components in parts by weight: 3-50 parts of radix et rhizoma rhei, 3-50 parts of moutan bark, 5-30 parts of peach seeds, 10-50 parts of of Chinese waxgourd seeds and 5-20 parts of mirabilite. The traditional Chinese medicine preparation has the advantages that the firstly provided traditional Chinese medicine preparation can integrally promote multiplication of lactobacillus in different periods, shortens the delay period of cultures, and prolongs rapid multiplication growth logarithmic phase, a high-level multiplication stable phase is maintained, the integral multiplication level and the number of the cultures are increased, enlargement and multiplication of cultures are facilitated, and the yield of metabolite L-lactic acid which is beneficial for physical health and generated by the cultures can be improved obviously. Therefore, the effects of the traditional Chinese medicine preparation on promoting multiplication of lactobacillus bulgaricus and secretion of lactic acid have good application prospects.
Owner:李超群

Fe2+ ion for promoting proliferation of paenibacillus polymyxa and application method thereof

The invention provides Fe2+ ion for promoting the proliferation of paenibacillus polymyxa and the application method thereof. An adopted culture medium is formed by adding Fe2+ into a PDB culture medium till the concentration is 50mg / L, and is equal to FeSO4.7H2O with the final concentration of 248.21mg / L. The Fe2+ ion can improve the proliferation biomass of paenibacillus polymyxa, shorten the proliferation lag phase of paenibacillus polymyxa, and ensure that the log phase of proliferation is in advance. The Fe2+ ion has an important theoretical significance on high efficient culture of paenibacillus polymyxa, and has a wide promotion value for the application of relevant biological bacterium agents.
Owner:JILIN AGRICULTURAL UNIV
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