Saline-alkaline tolerant bacillus siamensis strain and production method and application of live bacteria preparation of saline-alkaline tolerant bacillus siamensis strain
A technology of bacillus and live bacteria preparations, applied in the field of agricultural biology, can solve problems such as limited control effects, achieve scientific production methods, strong salt and alkali tolerance, and reduce the occurrence of rice bakanae disease
- Application Information
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Problems solved by technology
 Example 1: Screening of Bacillus siamese PAPM-15
 (1) Screening of saline-alkali-tolerant strains
 Bacillus siamese PAPM-15 was isolated from rice rhizosphere soil. The soil was sampled in moderately saline-alkali soil in Bincheng District, Binzhou City, Shandong Province in September 2018. The specific separation method is as follows: mix the soil sample evenly, weigh 5g, put it into a conical flask filled with 95mL sterile water and 10 glass beads, shake it at 37°C and 180rpm for 30min. Take 1 mL of soil suspension for 10 -1 -10 -7 Serial concentration gradient dilution, and then take 10 -5 、10 -6 、10 -7 The three dilutions were spread on the plate containing the selection medium, and cultured upside down at 37°C for 2 days. Single colonies were picked and streaked onto plates containing selection medium, and cultured upside down at 37°C for 2 days. Pick a single colony and transfer it to the slant of the preservation medium test tube, culture ...
 The identification of embodiment 2 Bacillus siamese PAPM-15
 (1) Morphological and physiological and biochemical characteristics
 The morphological characteristics of the Bacillus siamese PAPM-15 strain are: cultivated on NA medium at 37°C for 48h, the diameter of the colony is about 0.5-0.8mm, white and opaque, round, with irregular edges, rough surface, bulges, and wrinkles ; Bacteria are rods, such as figure 1 shown. The NA medium is nutrient agar medium, and its formula is: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH 7.0.
 Physiological and biochemical characteristics of the Bacillus siamese PAPM-15 strain: Gram-positive bacilli; positive catalase test, positive starch hydrolysis test, positive nitrate reduction test, positive malonate utilization test, positive gelatin liquefaction test , The methyl red test was negative, the D-glucose fermentation test was positive, the D-mannitol fermentation test was positive, and...
 The preparation of embodiment 3 bacillus siamese PAPM-15 microbial agent
 The preparation method of described bacillus siamese PAPM-15 strain microbial agent comprises the following steps:
 1) Activation of strains: Transfer the low-temperature-preserved Bacillus siamese PAPM-15 strains to the slant of NA medium test tubes, and culture them at 32-37° C. for 24-36 hours for activation. The NA medium is nutrient agar medium, and its formula is: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH7.0.
 2) Triangular flask seed preparation: Scrape the activated Bacillus siamese PAPM-15 lawn with an inoculation loop, inoculate it in NB medium, and culture it at 32-37° C. for 24-36 hours. The NB medium is a nutrient broth medium, and its formula is: 3 g of beef extract, 10 g of peptone, 5 g of NaCl, 1000 mL of water, and pH 7.0.
 3) Preparation of seed tank strains: Transfer the seeds of the triangular flask to a 10L seed tank eq...
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