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Ferment device of solid ferment aseptic manipulation

A technology of solid-state fermentation and aseptic operation, which is applied in solid-phase fermentation bioreactors, biochemical cleaning devices, enzymology/microbiology devices, etc. Achieve the effect of solving the problem of contamination, shortening the delay period, and solving the problem of easy contamination by manual operation

Inactive Publication Date: 2005-09-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to overcome the shortcomings of solid-state fermentation process and equipment because of its easy to infect bacteria, fermentation conditions are difficult to control, and industrial scale-up is difficult; in order to shorten the delay period and seed production cycle of the solid-state fermentation process, avoid infection of bacteria in the fermentation process; Thereby the present invention provides a kind of fermenting method and special-purpose device thereof of aseptic operation of solid-state fermentation

Method used

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  • Ferment device of solid ferment aseptic manipulation
  • Ferment device of solid ferment aseptic manipulation

Examples

Experimental program
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Effect test

Embodiment 1

[0033]According to Fig. 1, make a set of fermentation special-purpose device that adopts the aseptic operation of liquid-solid combination, comprising: a standard liquid deep layer standard fermenter as liquid seed tank 1; Inoculation cylinder 4 and solid-state fermentation reactor 13; Solid medium sterilization Tank 2, the solid medium sterilization tank is a pressure vessel equipped with a heating or cooling jacket, and can rotate up and down at 4-10 rpm, and the top and bottom of the tank are conical; mechanical drum sieve inoculation cylinder 7, culture shallow Disc 8, honeycomb cultivator 9, conveyor belt cultivator 10, class 100 ultra-clean room 11 and class 100 ultra-clean operation platform 12 are processed and manufactured according to EMP standard; wherein liquid seed tank 1 passes through pipeline 14 with valve and solid Medium sterilizing tank 2 communicates, and the wall of solid medium sterilizing tank 2 is connected with a pipeline 15 with a valve, and the lower ...

Embodiment 2

[0035] The seed liquid prepared by the liquid fermenter 1, the pressure of the liquid fermenter is increased by sterile air, and the seed liquid is transported to the solid medium sterilization tank 2 after autoclaving; the solid medium is sterilized at 120° C. for 30 minutes, and then , cooling the medium to 30--40°C; solid medium sterilizing tank 2 rotates 3-6 times, so that the liquid seed liquid and solid medium materials are evenly mixed; in the 100-level ultra-clean operation platform 12, quickly culture the solid The lower cover of the base sterilization tank 2 and the upper cover of the sterile inoculation tube 4 are opened, and the inner cloth tube 5 of the sterile inoculation tube is connected to the lower cover of the solid medium sterilization tank 2, and the uniformly mixed materials in the solid medium sterilization tank Drop vertically into the aseptic inoculation cylinder 4; in the 100-class clean room 11, open the lower cover of the aseptic inoculation cylinder...

Embodiment 3

[0039] The operation process of this embodiment is the same as that of Embodiment 1. The difference is that the solid-state fermentation culture is carried out in the solid-state fermentation reactor in the form of a conveyor belt-loaded closed pressure vessel after steam sterilization from a mechanical traction conveyor-type incubator.

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Abstract

The bacteria-free solid fermentation apparatus includes a liquid seed tank, a solid culture medium sterilizing tank with lower cover connected to the liquid seed tank via pipeline with valve, a 100-level superclean operation platform connecting the lower port of the solid culture medium sterilizing tank and the inoculating tube, a 100-level superclean chamber with mechanical vibrating sieve and culture shallow tray, honeycomb culture device or conveyer belt type culture device, and a solid fermentation reactor with air inlet connected via a tractor to the culture device. The bacteria-free manipulation process includes preparing seed, sterilizing culture medium, bacteria-free inoculation, bacteria-free charging, bacteria-free solid fermentation and other links to prevent bacteria pollution in solid fermentation.

Description

technical field [0001] The invention relates to a fermentation method and a device thereof, in particular to a fermentation method for aseptic operation of solid-state fermentation and a special device thereof. Background technique [0002] Solid-state fermentation refers to the fermentation process of one or more microorganisms carried out in a state where the medium is solid and rich in water, but there is no or almost no free-flowing water. The substrate (substrate) is a solid mixture that is insoluble in water. It can not only provide the carbon source, nitrogen source, inorganic salt, water and other nutrients required by microorganisms, but also a place for microbial growth. The most prominent advantages of solid-state fermentation are simple process, low investment, and high output. Its specific characteristics can be summarized as follows: (1) Low fermentation cost, cheap raw materials, and direct use of industrial and agricultural residues (renewable resources of ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12MC12M1/00C12M1/16
Inventor 陈洪章李佐虎
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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