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Method for decomposing cellulose to produce hydrogen with cellulose clostridium

A technology of cellulose and Clostridium, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long cultivation time, low hydrogen production rate, low cellulose utilization rate, etc., and achieve simple operation, manageable effects

Inactive Publication Date: 2009-04-08
HARBIN INST OF TECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of long cultivation time, low cellulose utilization rate and low hydrogen production rate in the existing method of using Clostridium cellulosicum H10 to decompose cellulose and ferment hydrogen production, and to provide a method for decomposing cellulose by Clostridium cellulosicum H10. Method for producing hydrogen from cellulose

Method used

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Examples

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specific Embodiment approach 1

[0008] Specific embodiment one: the method for the hydrogen production of Clostridium cellulosicum decomposing cellulose in this embodiment is realized according to the following steps: 1. The cell concentration is 1×10 10 Each / L Clostridium cellulosicum H10 bacteria solution is inoculated in the growth medium at a volume ratio of 8% to 12%, and the temperature is 35°C, the pH value is 7.0 to 8.0, and the mass concentration is 99.99% in a nitrogen atmosphere. Cultivate under conditions for 46-50 hours; 2. Transfer the cultured Clostridium cellulosicum H10 to the hydrogen-producing medium at a volume ratio of 8%-12%, and shake it at a temperature of 34°C and a pH of 7.0-8. The anaerobic fermentation is carried out in a nitrogen atmosphere with a bed speed of 90-110r / min and a mass concentration of 99.99% for 22-26 hours to complete the hydrogen production by Clostridium cellulosicum decomposing cellulose; wherein every 1L of the growth medium in step 1 The medium consists of 2....

specific Embodiment approach 2

[0011] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in step one, it is inoculated in the growth medium with a volume ratio of 9% to 11%. Cultivate for 47-49 hours under the condition of 99.99% nitrogen atmosphere. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0012] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step one, it is inoculated in the growth medium at a volume ratio of 10%, and the temperature is 35°C, the pH value is 7.5, and the mass concentration is 99.99% nitrogen. Incubate for 48 hours under atmospheric conditions. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

A method for using clostridium cellulosic to decompose cellulose and produce hydrogen relates to the method for decomposing the cellulose and producing the hydrogen. The method can solve the problems of long culture time, low utilization ratio of the cellulose and low hydrogen production rate of the prior method for utilizing the clostridium cellulosic H10 to decompose the cellulose and produce the hydrogen by fermentation. The method is as follows: 1. the clostridium cellulosic H10 is inoculated in a growth culture medium for culture; and 2. the clostridium cellulosic H10 after the culture is trans-inoculated in a hydrogen production culture medium for carrying out the anaerobic fermentation, thereby completing the decomposition of the cellulose and the hydrogen production by using the clostridium cellulosic. The delay period of the method is shortened by 20 percent to 30 percent, the utilization rate of the cellulose can be up to 65 percent to 75 percent and the hydrogen production rate achieves 1.9 plus or minus 0.1molH2 / mol hexose.

Description

technical field [0001] The invention relates to a method for decomposing cellulose to produce hydrogen. Background technique [0002] Biological hydrogen production technology uses microorganisms to ferment organic wastewater, straw-like agricultural and forestry waste, and organic waste to produce hydrogen, which has the characteristics of pollution control, environmental protection, and production capacity. Among them, the use of straw-like agricultural and forestry wastes to produce hydrogen has high efficiency, low energy consumption, less pollution, low cost, and great development potential. It has practical significance for the treatment of agricultural and forestry organic wastes and the sustainable development of resources, energy, environment and economy. . [0003] Clostridium cellulosicum H10 has strong cellulose metabolism ability, and the utilization rate of cellulose can reach 40% to 60%. At present, in the method of using Clostridium cellulosicum H10 to decom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
CPCY02E50/343Y02E50/30
Inventor 邢德峰王胜男任南琪
Owner HARBIN INST OF TECH
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