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Method for producing organic acid at high production rate through fermentation of intermittent backflow cells

A high-productivity, organic acid technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of long fermentation cycle and low productivity, improve productivity, save tank washing and sterilization time, The effect of good industrial production application value

Inactive Publication Date: 2015-04-15
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the outstanding problems of long fermentation cycle and low productivity in organic acid fermentation production, the present invention uses intermittent reflux of cells and instant replenishment of fresh medium to maintain high biomass in the fermentation process and achieve the purpose of producing organic acids with high productivity

Method used

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  • Method for producing organic acid at high production rate through fermentation of intermittent backflow cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Fermentative production of lactic acid

[0034] Equipment: one set of 100L seed tank, one set of 100L fermenter, one set of seed medium microfiltration device with processing capacity of 100L / h (membrane pore size 0.22μm), one set of fermentation medium microfiltration device with processing capacity of 100L / h ( Membrane pore size 0.22μm), a set of fermentation broth microfiltration device with a processing capacity of 100L / h (membrane pore size 0.22μm), a set of 100L medium buffer storage tank, a set of fermentation broth buffer storage tank, and five pumps.

[0035]Species: Lactobacillus delbrueckii (L. delbrueckii HG 07).

[0036] Seed medium (g / L): Adjust the concentration of reducing sugar to 50% in corn saccharification solution, add 10% of bran hydrolysis filtrate, 3% of yeast powder, and 3% of beef extract, mix well, and adjust the pH to 7.0 with 10mol / L NaOH solution.

[0037] Fermentation medium (g / L): Adjust the reducing sugar concentration to 100 in the cor...

Embodiment 2

[0044] Fermentative production of lactic acid

[0045] Equipment: one set of 100L seed tank, one set of 100L fermenter, one set of seed medium microfiltration device with processing capacity of 100L / h (membrane pore size 0.22μm), one set of fermentation medium microfiltration device with processing capacity of 100L / h ( Membrane pore size 0.22μm), a set of fermentation broth ultrafiltration device with a processing capacity of 100L / h (molecular weight cut-off 1000 Daltons), a set of 100L medium buffer storage tank, a set of fermentation broth buffer storage tank, and five pumps.

[0046] Strain: Lactobacillus rhamnosus (L. rhamnosus HG 08).

[0047] Seed medium (g / L): Adjust the reducing sugar concentration to 10 in the rice saccharification solution, add bran hydrolysis filtrate 3, yeast powder 1, and beef extract 1, mix well, and adjust the pH to 7.0 with 8mol / L KOH solution.

[0048] Fermentation medium (g / L): Adjust the reducing sugar concentration to 100 in the rice sacch...

Embodiment 3

[0055] Fermentative production of lactic acid

[0056] Equipment: one set of 100L seed tank, one set of 100L fermenter, one set of seed medium microfiltration device with processing capacity of 100L / h (membrane pore size 0.22μm), one set of fermentation medium microfiltration device with processing capacity of 100L / h ( Membrane pore size 0.22μm), a set of fermentation broth microfiltration device with a processing capacity of 100L / h (membrane pore size 0.22μm), a set of 100L medium buffer storage tank, a set of fermentation broth buffer storage tank, and five pumps.

[0057] Strains: Lactobacillus casei (L. casei LZD12).

[0058] Seed medium (g / L): Adjust the concentration of reducing sugar to 50% in corn saccharification solution, add 10% of bran hydrolysis filtrate, 3% of yeast powder, and 3% of beef extract, mix well, and adjust the pH to 7.0 with 10mol / L NaOH solution.

[0059] Fermentation medium (g / L): Adjust the reducing sugar concentration to 100 in the corn saccharif...

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Abstract

The invention provides a method for producing organic acid at a high production rate through fermentation of intermittent backflow cells, which comprises the following steps of: performing filtration sterilization on a fresh culture medium, introducing into a fermentation tank, and inoculating high-density seed liquor into the fermentation tank; controlling organic acid fermentation production conditions of the used strain, when fermentation is performed until a small amount of sugar residue is in fermentation liquor, discharging partial or all fermentation liquor to a filtering device for processing, simultaneously supplementing a fresh germ-free culture medium into the fermentation tank, returning intercepted strain cell concentrated solution to the fermentation tank to serve as a production strain for later use, wherein the filtered liquor is used for extracting organic acid; and only when the production rate of the organic acid is obviously reduced, not returning the strain cell concentrated solution intercepted by the filtering device is not to the fermentation tank any more, and supplementing new high-density seed liquor and a fresh germ-free fermentation culture medium into the fermentation tank so as to perform a new round of production of organic acid at the high production rate through fermentation of the intermittent backflow cells. Through the method, high biomass and high production rate are always kept in the organic acid fermentation production process, and the method has high industrial application value.

Description

Technical field: [0001] The invention belongs to the field of biological process engineering, and in particular relates to a method for producing organic acids by intermittent high-productivity fermentation of reflux cells. Background technique: [0002] Organic acids refer to some organic compounds with acidity. The most common organic acids are carboxylic acids, which are industrial raw materials for organic synthesis, industrial and agricultural production and medicine. At present, lactic acid and various amino acids in organic acids are used more and more widely. For example, lactic acid is an organic acid with a wide range of uses, which can be used in brewing, medicine, leather, cigarettes, chemicals, food, printing and dyeing, etc. The market The demand is very large, especially as the main raw material for the production of a new biodegradable polymer material - polylactic acid (PLA), which has a large potential market demand. Amino acids are widely used in the fiel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12P13/10C12P13/22C12R1/13C12R1/15C12R1/245C12R1/225
Inventor 余龙江卢正东鲁明波何峰
Owner HUAZHONG UNIV OF SCI & TECH
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