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A Salt-alkali-tolerant Pacific Bacillus and Its Live Bacterial Preparation and Application

A technology of bacillus and live bacteria preparation, applied in the field of agricultural biology, can solve problems such as limited control effect, and achieve the effects of reducing disease occurrence, improving biological stability, and strong inhibitory effect

Active Publication Date: 2021-01-19
SHANDONG ZOETICLAND BIOLOGICAL TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, beneficial microorganisms such as Bacillus cereus, Trichoderma harzianum, Bacillus subtilis, Bacillus thuringiensis, Streptomyces lavender, Streptomyces griseus and other beneficial microorganisms have been used in the biological control of cabbage soft rot, but due to the limited control effect, they have not been Large-scale promotion in agricultural production

Method used

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  • A Salt-alkali-tolerant Pacific Bacillus and Its Live Bacterial Preparation and Application
  • A Salt-alkali-tolerant Pacific Bacillus and Its Live Bacterial Preparation and Application
  • A Salt-alkali-tolerant Pacific Bacillus and Its Live Bacterial Preparation and Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019]Example 1: Screening of Pacific Bacillus YJJK-16

[0020](1) Screening of salt-tolerant strains

[0021]Pacific Bacillus YJJK-16 was isolated from the rhizosphere soil of cabbage. The soil was sampled in Dongying City, Shandong Province, as saline soil. The specific separation method is as follows: Weigh 10 g of soil sample, put it into a pre-sterilized 250 mL conical flask containing 90 mL of deionized water and 10-20 glass beads, and shake at 180 r / min for 20 minutes. Using gradient dilution method, dilute the soil solution to 10-2, 10-3, 10-4, 10-5, 10-6, 10-7. Pipette 0.1 mL of soil solution of different dilutions, put it into a high-salt-alkali selective medium plate, spread evenly, and then incubate at 37°C for 24-48h. According to the difference of colony and cell morphology, different strains were transferred to the slant of the preservation medium test tube, cultured at 37°C for 1-2 days, covered with moss, and stored in a refrigerator at 4°C for later use. A total of 82 sa...

Embodiment 2

[0027]Example 2: Identification of Pacific Bacillus YJJK-16

[0028](1) Morphological and physiological and biochemical characteristics

[0029]The colony and bacterial characteristics of the Pacific Bacillus sp. YJJK-16 strain: cultured on LB medium at 37°C for 2 days, the colony was milky white, 1.5-2.5mm in diameter, translucent, bulging, smooth and moist, with regular edges. The texture is relatively uniform; the bacteria are rod-shaped with spores. The LB medium is Luria-Bertani medium, and its formula is: 5 g of yeast powder, 10 g of tryptone, 10 g of sodium chloride, 20 g of agar, 1000 mL of water, and pH 7.0.

[0030]The physiological and biochemical characteristics of the Pacific Bacillus sp. YJJK-16 strain: Gram stain test positive, contact enzyme test positive, starch hydrolysis test positive, exercise test positive, nitrate reduction test positive, gelatin liquefaction test positive, D-glucose The acid production test is positive, the anaerobic growth test is negative, the acetyl...

Embodiment 3

[0034]Example 3: Preparation of Bacillus Pacificus YJJK-16 inoculum

[0035]The preparation method of the Pacific Bacillus YJJK-16 strain inocula includes the following steps:

[0036]1) Strain activation: Transfer the cryopreserved Bacillus pacificum YJJK-16 strain to the slope of the LB medium test tube, and culture it at 37°C for 24 hours for activation. The LB medium is Luria-Bertani medium, and its formula is: 5 g of yeast powder, 10 g of tryptone, 10 g of sodium chloride, 20 g of agar, 1000 mL of water, and pH 7.0.

[0037]2) Preparation of Erlenmeyer flask seeds: scrape the activated Bacillus Pacificus YJJK-16 lawn with an inoculating loop, inoculate it in LB medium, and cultivate it at 37°C for 24 hours. The LB medium is Luria-Bertani medium, and its formula is: 5 g of yeast powder, 10 g of tryptone, 10 g of sodium chloride, 20 g of agar, 1000 mL of water, and pH 7.0.

[0038]3) Preparation of seed tank strains: transfer the Erlenmeyer flask seeds to a 10L seed tank containing 6L LB med...

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Abstract

The invention discloses a saline-alkali-tolerant pacific bacillus and its live bacterial preparation and application. A strain of Bacillus pacificus YJJK‑16 isolated from the rhizosphere soil of cabbage in saline-alkali land was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on July 26, 2019, with the preservation number CGMCC No.18300. Add propyl gallate, biochemical fulvic acid powder and cassonne to the fermented liquid of the bacterial strain and stir evenly to obtain live bacterial preparation. Bacillus pacificus YJJK‑16 has strong saline-alkali tolerance, has a high colonization rate in saline-alkali soil, and has a strong antagonistic effect on cabbage soft rot pathogens. It can be used to produce microbial fertilizers, especially microbial fertilizers for cabbage in saline-alkali soils. It is used in the biological control of cabbage soft rot, reducing the occurrence of diseases and improving yield and quality.

Description

Technical field[0001]The invention relates to a saline-tolerant Pacific bacillus and its application in preventing and treating soft rot of cabbage, belonging to the field of agricultural biotechnology.Background technique[0002]Chinese cabbage is also called yellow sprout and Chinese cabbage. It is a Brassica genus of the cruciferous family. It is widely cultivated in my country and is known as the "king of a hundred vegetables". Soft rot has always been one of the key factors restricting the high-yield and high-quality cultivation and large-scale development of Chinese cabbage. It is common in all parts of the country and the damage period is very long. It can cause cabbage rot in the field, during storage and transportation, and even on the market, causing very serious losses. The yield loss can reach 30%-50%, and the storage loss can reach about 20%.[0003]The pathogen of soft rot belongs to the genus Erwinia (Erwinia), which is divided into three groups, namely, fire blight (amyl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 孙中涛辛寒晓刘丽英赵升远孙国科杨越超史庆华候汉学寇娟妮刘珂欣
Owner SHANDONG ZOETICLAND BIOLOGICAL TECH
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