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Culture medium with composite trace elements and capable of promoting proliferation of paenibacillus polymyxa and application method of culture medium

A technology of compounding trace elements and polymyx spore-like spores, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effects of reducing pesticide residues, wide antibacterial spectrum and high efficiency

Inactive Publication Date: 2019-12-10
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the compounding of trace elements has different effects on promoting the proliferation of different strains, and Fe 2+ 、Cu 2+ , Zn 2+ , Mn 2+ Study on the Proliferation of Paenibacillus polymyxa by Ion Compounding

Method used

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  • Culture medium with composite trace elements and capable of promoting proliferation of paenibacillus polymyxa and application method of culture medium
  • Culture medium with composite trace elements and capable of promoting proliferation of paenibacillus polymyxa and application method of culture medium
  • Culture medium with composite trace elements and capable of promoting proliferation of paenibacillus polymyxa and application method of culture medium

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Embodiment 1

[0008] Embodiment 1 A kind of culture medium and application method thereof for promoting the proliferation of Paenibacillus polymyxa by adding composite trace elements

[0009] 1. Materials and methods

[0010] 1.1 Materials

[0011] Paenibacillus polymyxa in ginseng ( Paenibacillus polymyxa ), isolated and identified in our laboratory, was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on February 5, 2013, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, The deposit number is CGMCC 7250; potato, glucose, CuSO 4 ·5H 2 O, FeSO 4 ·7H 2 O, ZnSO 4 ·7H 2 O, MnSO 4 ·H 2 O, pure water, and the remaining reagents are of analytical grade.

[0012] 1.2 Instruments and equipment

[0013] CHA-S air bath reciprocating constant temperature shaking incubator; Tecan Infinite® 200 Pro multifunctional microplate reader.

[0014] 1.3 Metho...

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Abstract

The invention provides a culture medium with composite trace elements and capable of promoting proliferation of paenibacillus polymyxa and an application method of the culture medium, and provides appropriate concentration of the composite trace elements in the culture medium and a specific application method of the culture medium. The proliferation biomass of the paenibacillus polymyxa can be increased, the proliferation delaying phase of the paenibacillus polymyxa can be shortened, and the logarithmic phase of the proliferation can be advanced. The culture medium disclosed by the invention has important theoretical significance in high-efficiency culture of the paenibacillus polymyxa, and has high popularization value in the application of relevant biological microbial agents.

Description

technical field [0001] The invention relates to a culture medium for promoting the proliferation of Paenibacillus polymyxa by adding compound trace elements and an application method thereof, which belongs to the technical field of biological pesticides and biological medicines. Background technique [0002] Paenibacillus polymyxa in ginseng has a variety of physiological and pharmacological activities. It can produce a series of biologically active substances, such as nitrogenase, plant hormones, polysaccharides, hydrolytic enzymes, antibiotics, etc., which have great application value in biological nitrogen fixation, promotion of plant growth, development of new antibiotics, and inhibition of plant diseases. With the increase of environmental pressure and the improvement of people's health and food safety requirements, microbial pesticides have received more and more attention. The present invention adopts the ginseng endogenous Paenibacillus polymyxa strain isolated from...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/12
CPCC12N1/20
Inventor 郜玉钢臧埔赵岩祝洪艳何忠梅王立岩肖茹雪张连学
Owner JILIN AGRICULTURAL UNIV
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