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Method for promoting proliferation of paenibacillus polymyxa through Zn2+ ions and application method of paenibacillus polymyxa

A technology of sporogenous polymyxa and its application method, which is applied in the fields of biopesticides and biomedicine, and can solve the problems of different effects of promoting proliferation, etc.

Inactive Publication Date: 2018-09-28
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Visible, Zn 2+ Different strains have different effects on promoting proliferation, Zn has not been seen 2+ Study on the Proliferation Effect of Ions on Paenibacillus Polymyxa

Method used

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  • Method for promoting proliferation of paenibacillus polymyxa through Zn2+ ions and application method of paenibacillus polymyxa

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Experimental program
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Effect test

Embodiment 1

[0005] Embodiment 1 a kind of Fe 2+ Ions promote the proliferation of Paenibacillus polymyxa and its application method

[0006] 1. Materials and methods

[0007] 1.1 Materials

[0008] The bacterial classification used in the present invention is endophytic Paenibacillus polymyxa of ginseng ( Paenibacillus polymyxa ), which was deposited on February 5, 2013 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 7250. Glucose, ZnSO 4 ·7H 2 O (Beijing Chemical Industry). Purified water was from Hangzhou Wahaha Company; other reagents were of analytical grade.

[0009] 1.2 Instruments and equipment

[0010] CHA-S air bath reciprocating constant temperature shaking incubator; Tecan Infinite® 200 Pro multifunctional microplate reader.

[0011] 1.3 Method

[00...

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Abstract

The invention provides a method for promoting proliferation of paenibacillus polymyxa through Zn2+ ions and an application method of paenibacillus polymyxa. According to the method, a culture medium is prepared in the mode that Zn2+ is added to a PDB culture medium until the final concentration of the PDB culture medium is 100 mg / L, and the culture medium is equivalent to ZnSO4.7H2O with the concentration of 439.75 mg / L. According to the method, the biomass of proliferation of paenibacillus polymyxa can be increased, the delay period of proliferation of paenibacillus polymyxa is shortened, andthe logarithmic phase of proliferation is shortened. The method has important theoretical significance on efficient culture of paenibacillus polymyxa and has high popularization value on applicationof related antibiological inoculants.

Description

technical field [0001] The present invention relates to a kind of Zn 2+ The ion promotes the proliferation of Paenibacillus polymyxa and its application method, belonging to the technical field of biopesticides and biomedicine. Background technique [0002] Paenibacillus polymyxa has a wide range of physiological and pharmacological activities. It is a probiotic with various biological activities such as improving plant resistance, promoting plant growth, absorbing heavy metals, anti-oxidation and anti-tumor. It can produce a series of biologically active substances. Such as nitrogenase, plant hormones, polysaccharides, hydrolytic enzymes, antibiotics, etc., have great application value in biological nitrogen fixation, promotion of plant growth, development of new antibiotics, and inhibition of plant diseases. Paenibacillus polymyxa is also a first-class strain exempted from safety inspection by the Ministry of Agriculture of my country, and its development and application ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12R1/01
CPCC12N1/20C12N1/38
Inventor 郜玉钢赵岩臧埔肖茹雪祝洪艳何忠梅王立岩张连学
Owner JILIN AGRICULTURAL UNIV
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