A kind of bacillus cereus and its preparation and application
A technology of Bacillus cereus and preparations, applied in the field of microorganisms, can solve the problems of low efficiency of oxytetracycline and achieve the effects of shortening the degradation time of oxytetracycline, high selection efficiency, and high degradation efficiency
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[0054] The preparation method of bacillus cereus preparation provided by the invention comprises the following steps:
[0055] (a) Put the Bacillus cereus seed liquid into the culture medium of the fermenter and cultivate it, the inoculum amount is 2%-5%, until the spore formation is no less than 90%, and the fermentation liquid is obtained.
[0056] The configuration of the fermentor culture medium is as follows: soybean meal 6-7%, corn flour 7-8%, bran 1%-2%, humic acid 0.5%-1%, NaH 2 PO 4 0.05%~0.1%, K 2 HPO 4 0.05%~0.1%, (NH 4 ) 2 SO 4 0.1% to 0.2%, adjust the pH value to 7.0 to 7.4, and sterilize at high temperature and high pressure for 15 to 30 minutes.
[0057] The Bacillus cereus seed liquid is prepared by multistage cultivation with the Bacillus cereus strain, and the specific method is as follows:
[0058] (I) First-class seeds: Inoculate the strains in a 500mL Erlenmeyer flask, the liquid volume is 150mL, the inoculum volume is 4-5%, 35-37°C, 120-180rpm shak...
Embodiment 1
[0071] A screening method for highly efficient oxytetracycline-degrading bacteria, comprising the following steps:
[0072] (1) Single colony culture:
[0073] The pig manure contaminated by oxytetracycline was taken to prepare a bacterial suspension, and the bacterial suspension was added to the basal medium. After the culture medium was solidified, the culture dish was inverted and cultured in a constant temperature incubator at 35°C for 3 days. After that, typical colonies with different shapes and clear colonies were selected for culture, and a single bacterial colony was cultivated.
[0074] The basal medium is prepared as follows: accurately weigh 10.0 g of peptone, 3.0 g of beef extract, 5.0 g of sodium chloride, and 15 g of agar, heat and dissolve in 1 L of distilled water, adjust the pH to 7.0, and sterilize at high temperature and high pressure for 15 minutes ; After the culture medium was cooled to 48° C., filter-sterilized cycloheximide was added so that the conce...
Embodiment 2
[0082] A screening method for highly efficient oxytetracycline-degrading bacteria, comprising the following steps:
[0083] (1) Single colony culture:
[0084] The pig manure contaminated by oxytetracycline was taken to prepare a bacterial suspension, and the bacterial suspension was added to the basal medium. After the culture medium was solidified, the culture dish was inverted and cultured in a constant temperature incubator at 35°C for 3 days. After that, typical colonies with different shapes and clear colonies were selected for culture, and a single bacterial colony was cultivated.
[0085]The basal medium is prepared as follows: accurately weigh 12.0 g of peptone, 5.0 g of beef extract, 6.0 g of sodium chloride, and 20 g of agar, heat and dissolve them in 1 L of distilled water, adjust the pH to 7.4, and sterilize at high temperature and high pressure for 30 minutes ; After the culture medium was cooled to 50° C., filter-sterilized cycloheximide was added so that the c...
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