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A strain of saline-alkali-tolerant Streptomyces ochreus and its application

A technology of Streptomyces yellow ochre and bacterial agent, which is applied in the field of agricultural microorganisms and ecological restoration, can solve more than 20 kinds of problems such as P. However, it is isolated from the cotton rhizosphere soil of saline-alkali land, and can degrade phenylallylic acid, p-hydroxybenzoic acid and ferulic acid salt-tolerant Streptomyces yellow ochre and other problems at the same time, so as to improve the utilization rate of raw materials, low production cost, The effect of improving stability

Active Publication Date: 2020-11-03
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to reports, although many microorganisms have the ability to degrade phenolic acids, including more than 20 species such as Phanerochaete chrysosporium, Serratia marcescens, Azospirillum adipogenicum, and Bacillus amyloliquefaciens, but isolated from The salt-tolerant Streptomyces ochreus in the cotton rhizosphere soil in saline-alkali land, which can simultaneously degrade phenylacrylic acid, p-hydroxybenzoic acid, ferulic acid and other phenolic acids, has not been reported yet.

Method used

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  • A strain of saline-alkali-tolerant Streptomyces ochreus and its application
  • A strain of saline-alkali-tolerant Streptomyces ochreus and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the isolation of bacterial strain

[0053] The strain was isolated from cotton rhizosphere soil in saline-alkali land. The soil was sampled in Yongan Town, Kenli District, Dongying City, Shandong Province, which was a saline-alkali soil where cotton had been planted continuously for more than 5 years.

[0054] The specific separation method is as follows: weigh 5 g of the mixed soil sample, put it into 100 mL of enrichment medium, and shake and culture at 37 ° C and 150 rpm for 7 days. Take 1 mL of the enriched culture for 10 -1 ~10 -5 Serial dilution, then take 10 -2 、10 -3 、10 -4 The three dilutions were spread on the plate containing the selection medium, and cultured at 37°C for 7 days. Single colonies were picked and streaked onto plates containing selection medium, and cultured at 37°C for 7 days. Pick a single colony and transfer it to the slant of the preservation medium test tube, culture it at 37°C for 7 days, and after it is covered with ...

Embodiment 2

[0061] Embodiment 2: identification of strain

[0062] 1. Morphological and physiological and biochemical characteristics:

[0063] The morphological characteristics of the PAPM18 bacterial strain are: a single bacterial colony is flat, round or nearly round on Gao's No. 1 medium, and the surface is dry. The colonies were initially white and gradually turned pale yellow. The central ring is depressed, and there is a concentric ring pattern on the outer edge. The edge is relatively smooth, slightly irregular corrugated, and the back view is earthy yellow. The hyphae in the base have no transverse septa, the aerial filaments are flexible, the spores are straight or flexible, and the surface of the spores is smooth and oval.

[0064] The physiological and biochemical characteristics of the PAPM18 strain are: positive nitrate reduction test, positive starch hydrolysis test, positive D-glucose test, positive D-fructose test, positive sucrose utilization test, positive maltose uti...

Embodiment 3

[0075] Embodiment 3: Degradation test of Streptomyces yellow ochreus PAPM18 p-phenylacrylic acid, p-hydroxybenzoic acid, ferulic acid

[0076] Prepare the culture medium containing phenylacrylic acid, and its formula is: 1000 mg of phenylacrylic acid, 20 g of soluble starch, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 6g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH=7.4-7.6.

[0077] Prepare a medium containing p-hydroxybenzoic acid, its formula is: p-hydroxybenzoic acid 1000mg, soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 6g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH=7.4-7.6.

[0078] Prepare the culture medium containing ferulic acid, its formula is: ferulic acid 1000mg, soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 6g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH=7.4-7.6.

[0079] Streptomyces chrysogenum PAPM18 was transferred into the above-mentioned three mediums, and cultured with shaking at 37°C a...

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Abstract

The present invention discloses a strain of Streptomyces silaceus PAPM18, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on July 4, 2018, and its biological preservation number is: CGMCC NO.16054 . The strain can survive in saline-alkali soil, and has a good degradation effect on plant autotoxic substances such as phenylacrylic acid, p-hydroxybenzoic acid, and ferulic acid, and can reduce continuous cropping obstacles caused by them. Promote growth and increase yield. After expanding the culture, the strain was inoculated in a medium with cornstarch and corn steep liquor as the main nutrient source, cultured at 35-37°C for 72-96h, and then trehalose, molasses and biological fulvic acid powder were added to the fermentation broth, and then The live bacteria preparation can be obtained by spray drying. The preparation method of the microbial preparation is scientific, the fermentation period is short, the fermentation liquid bacterial concentration is high, the loss of live bacteria is small in the spray drying process, and the stability of the live bacteria preparation is high in the preservation process.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms and ecological restoration, in particular to a strain of saline-alkali-tolerant Streptomyces ochreus and application thereof. Background technique [0002] The total area of ​​saline-alkali land in my country is as high as more than 500 million mu, and it is of great significance to develop and utilize it reasonably. Commonly used saline-alkali land improvement methods include physical, chemical, water conservancy, biological and other measures, among which biological improvement has the most ecological benefits and development prospects. The application of biological fertilizers is a common method for biological improvement of saline-alkali soil, but whether the functional microorganisms in biological fertilizers can survive in saline-alkali soil directly affects its use effect. Therefore, it is of great significance to screen functional microorganisms that can tolerate salin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/28A01P21/00C05F11/08C12R1/465C09K101/00
CPCA01N63/00C05F11/08C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/465
Inventor 孙中涛刘丽英寇娟妮刘珂欣
Owner SHANDONG AGRICULTURAL UNIVERSITY
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