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Saline-alkaline-tolerant Bacillus aerius and preparation method and application of living bacteria preparation of saline-alkaline-tolerant Bacillus aerius

A technology of bacillus and live bacteria preparation, applied in the field of agricultural biology, can solve problems such as failure to popularize in a large area and limited control effect

Active Publication Date: 2020-10-23
德州八虎生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, beneficial microorganisms such as Trichoderma harzianum, Trichoderma viride, and Bacillus subtilis have been used for biological control of corn sheath blight, but due to limited control effects, they have not been widely used in agricultural production

Method used

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  • Saline-alkaline-tolerant Bacillus aerius and preparation method and application of living bacteria preparation of saline-alkaline-tolerant Bacillus aerius
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  • Saline-alkaline-tolerant Bacillus aerius and preparation method and application of living bacteria preparation of saline-alkaline-tolerant Bacillus aerius

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening of Bacillus aeruginosa strain PAPM-14

[0023] (1) Screening of salt-tolerant strains

[0024] Air Bacillus PAPM-14 was isolated from corn rhizosphere soil. The soil was sampled in Bincheng District, Binzhou City, Shandong Province in September 2018. It is a saline soil. The specific separation method is as follows: mix the soil sample, weigh 5 g, put it into a triangular flask containing 95 mL of sterile water and 10 glass beads, and shake at 37° C. and 180 rpm for 30 min. Take 1mL of the soil suspension for 10 -1 —10 -7 Serial concentration dilution, then take 10 -5 , 10 -6 , 10 -7 Three dilutions were spread on a plate containing selective medium, and incubated at 37°C for 2 days. Then, different single colonies were picked and streaked onto the plate containing the selection medium, and cultured upside down at 37°C for 2 days. Pick a single colony from the plate and transfer it to the slant surface of the preservation medium test tube, culture it ...

Embodiment 2

[0038] Example 2: Identification of Air Bacillus PAPM-14

[0039] (1) Morphological and physiological and biochemical characteristics

[0040] The morphological characteristics of the PAPM-14 strain are: cultured on NA medium at 37°C for 48 hours, the colonies are round, raised, moist, wrinkled, opaque, and milky white; the bacteria are short rod-shaped, such as figure 1 Shown. The NA medium is a nutrient agar medium, and its formula is: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH 7.0.

[0041] The physiological and biochemical characteristics of the PAPM-14 strain are: gram-positive coryneform bacteria; catalase test positive, citrate utilization positive, malonate utilization negative, nitrate reduction positive, VP test positive; starch The hydrolysis test was positive, the D-glucose fermentation test was positive, the D-mannitol fermentation test was positive, the lactose fermentation test was positive, and the maltose fermentation test was positive.

[0042...

Embodiment 3

[0045] Example 3: Functional verification of Bacillus aeruginosa PAPM-14 strain

[0046] (1) The ability of Bacillus aeruginosa PAPM-14 to produce IAA

[0047] Bacillus aeruginosa PAPM-14 was added to the LB liquid medium containing L-tryptophan (200mg / L), 37°C, 180r / min, and cultured on a shaker for 4 days. Take 50μL of bacterial suspension and drop it on the white ceramic plate, and add 50μLSalkowski colorimetric solution at the same time. Add 50μL of 50mg / L IAA colorimetric solution as the positive control CK. The white ceramic plate was placed at room temperature and protected from light for 30 minutes and observed. The color turned red indicates that IAA can be secreted. The color of the bacterial suspension of strain PAPM-14 was red after the experimental treatment, indicating that the strain PAPM-14 has a strong ability to secrete IAA ( image 3 ).

[0048] The formula of the LB liquid medium is: peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, distilled water 1000mL; sterili...

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Abstract

The invention discloses saline-alkaline-tolerant Bacillus aerius and a preparation method and application of a living bacteria preparation of the saline-alkaline-tolerant Bacillus aerius. According tothe invention, the saline-alkaline tolerant Bacillus aerius PAPM-14 is separated from rhizosphere soil of corn. The strain is preserved in China General Microbiological Culture Collection Center on June 22, 2020, and the preservation number of the strain is CGMCC No. 20118. The strain has strong salt and alkali tolerance; the strain has strong IAA production, phosphate solubilization and potassium solubilization capabilities, has a strong antagonistic effect on pathogenic bacteria such as corn sheath blight disease, can be used for producing microbial fertilizers, especially microbial fertilizers special for saline-alkali soil corn, is used for biological prevention and control of crop diseases such as corn sheath blight disease, reduces the occurrence of diseases, and improves the yieldand quality.

Description

Technical field [0001] The invention relates to a bacillus air which has the functions of salt-alkali tolerance, IAA production, phosphorus and potassium release and antagonism against corn sheath blight pathogens and its application, and belongs to the field of agricultural biotechnology. Background technique [0002] Corn is an annual gramineous plant of the genus Zea, widely distributed in the United States, China, Brazil and other countries, and is an important food crop in the world. Corn sheath blight is one of the important diseases that restrict the high-yield and high-quality corn cultivation. It is a disease caused by Rhizoctonia solani, Rhizoctonia zea and Rhizoctonia graminearum, among which Rhizoctonia solani More common. Maize sheath blight is common throughout the country, and the damage period is very long, from the seedling stage to the late growth stage, but it is more obvious from the tasseling stage to the filling stage, and it mainly damages the leaf sheath,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/60A01N63/22A01P3/00A01P21/00C12R1/07
CPCC12N1/20C05G3/00C05G3/60A01N63/22C12R2001/07C12N1/205
Inventor 孙中涛辛寒晓潘雁鹏赵升远孙国科陈君君安良聪
Owner 德州八虎生物科技有限公司
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