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Supplementary culture medium for shiitake mushroom fermentation and liquid submerged continuous culture method for shiitake mushrooms

The technology of feeding medium and culture method is applied in the field of deep layer continuous culture of shiitake mushrooms, which can solve the problems of low production efficiency of shiitake liquid batch fermentation, intolerance to shearing, and adherent growth, so as to improve the culture efficiency and biological volume, solving the effect of high viscosity and increasing yield

Inactive Publication Date: 2017-12-01
SHANDONG LUKANG SHELILE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the liquid batch fermentation of shiitake mushrooms tends to present technical problems such as high viscosity, adherent growth, and intolerance to shearing, which leads to low production efficiency of shiitake mushroom liquid batch fermentation

Method used

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  • Supplementary culture medium for shiitake mushroom fermentation and liquid submerged continuous culture method for shiitake mushrooms
  • Supplementary culture medium for shiitake mushroom fermentation and liquid submerged continuous culture method for shiitake mushrooms
  • Supplementary culture medium for shiitake mushroom fermentation and liquid submerged continuous culture method for shiitake mushrooms

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preparation example Construction

[0038] The present invention has no special limitation on the preparation method of the above-mentioned feed culture medium. By adopting conventional operations in the art, as long as the above-mentioned components can be formulated into the required mixed aqueous solution according to the specified ratio, the feed culture described in the present invention can be obtained. base.

[0039] The present invention also provides a continuous cultivation method of shiitake mushroom liquid, comprising the following steps: inoculating shiitake mushroom seed liquid in a liquid fermentation medium, fermenting and cultivating at a constant temperature at 22-28°C, and controlling the dissolved oxygen of the fermented liquid during the fermentation process. Below 25%, the pH value is 3.5~4.0; After fermentation for 8-12 days, add the feeding medium for mushroom fermentation described in the above technical scheme to the fermented liquid with the feeding rate of dilution rate 0.01-0.04 / h, co...

Embodiment 1

[0068] The weight percent of liquid fermentation medium components is as follows: glucose: 5%; polypeptone: 0.25%, yeast extract powder: 0.25%, potassium dihydrogen phosphate: 0.2%, magnesium sulfate: 0.1%, calcium chloride: 0.1%, The rest is tap water.

[0069] Seed bottle preparation: make 1000ml of liquid fermentation medium according to the recipe requirements, and pack into 500ml Erlenmeyer flasks, with a liquid volume of 100ml, and pack into 10 bottles. The mushroom spore liquid was inoculated into the Erlenmeyer flask by the spore liquid inoculation method, the temperature was controlled at 25° C., the rotational speed was 150 rpm, and the shaking culture was ready for use after 10 days.

[0070] Preparation of seed tanks: 30L tanks were used as seed tanks, and 20L medium was prepared as required, kept at 121°C and 110kpa for 50 minutes for sterilization, and cooled to 25°C after sterilization. The above-mentioned 1000ml seed liquid is inserted into the seed tank by me...

Embodiment 2

[0075] The weight percent of liquid fermentation medium components is as follows: glucose: 5%; polypeptone: 0.25%, yeast extract powder: 0.25%, potassium dihydrogen phosphate: 0.2%, magnesium sulfate: 0.1%, calcium chloride: 0.1%, The rest is tap water.

[0076] Seed bottle preparation: make 1000ml of liquid fermentation medium according to the recipe requirements, and pack into 500ml Erlenmeyer flasks, with a liquid volume of 100ml, and pack into 10 bottles. The mushroom spore liquid was inoculated into the Erlenmeyer flask by the spore liquid inoculation method respectively, the temperature was controlled at 25° C., the rotation speed was 150 rpm, and the shake culture was ready for use after 8 days.

[0077]Preparation of seed tanks: 30L tanks were used as seed tanks, and 20L medium was prepared as required, kept at 121°C and 110kpa for 50 minutes for sterilization, and cooled to 25°C after sterilization. The above-mentioned 1000ml seed liquid is inserted into the seed tan...

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Abstract

The invention belongs to the technical field of liquid submerged fermentation of edible fungi and particularly relates to a supplementary culture medium for shiitake mushroom fermentation and a liquid submerged continuous culture method for shiitake mushrooms. The supplementary culture medium is prepared from, by weight, 4-6% of glucose, 0.5-1% of polypeptone, 0.5-1% of yeast extract powder, 0.1-0.3% of monopotassium phosphate, 0.05-0.15% of magnesium sulfate, 0.05-0.15% of calcium chloride, 0.3-0.5% of soybean oil, 0.01-0.03% of tween 80, 0.05-0.15% of acetic acid and 91-93% of water. The supplementary culture medium is comprehensive and balanced in nutrition and capable of effectively prolonging an exponential growth phase of the shiitake mushrooms, improving liquid submerged culture efficacy of the shiitake mushrooms and increasing biomass and lentinan yield when being used for liquid submerged continuous culture of the shiitake mushrooms.

Description

technical field [0001] The invention belongs to the technical field of liquid submerged fermentation of edible fungi, and in particular relates to a feeding medium for fermenting and producing shiitake mushrooms and a method for continuously culturing liquid submerged shiitake mushrooms using the feeding medium. Background technique [0002] Lentinan (Lentinan) (molecular formula: (C 6 h 10 o 5 ) n ) is an effective active ingredient extracted from high-quality shiitake mushroom fruiting bodies. It has the functions of immune regulation, anti-tumor, anti-infection, anti-virus, anti-oxidation, anti-aging, and liver protection. It is mainly used in the fields of medicine and health products. Lentinan can enhance humoral immunity and cellular immunity, and can fight against influenza virus. It is an anti-influenza health food to be developed. [0003] The production of lentinan includes extraction from natural or artificially cultivated mushroom fruiting bodies and extracti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
CPCC12N1/14C12P19/04
Inventor 郭强申洋桑艳丽翟玥晗夏俊红
Owner SHANDONG LUKANG SHELILE PHARMA
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