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Salt-alkali-resistant streptomyces silaceus and application thereof

A technology of Streptomyces chrysanthemum and bacterial agents, applied in the field of agricultural microorganisms and ecological restoration, can solve more than 20 diseases such as Phanerochaete chrysosporium, Serratia marcescens, Azospirillum adipogeneticum, Bacillus amyloliquefaciens, etc. However, it is isolated from the rhizosphere soil of cotton in saline-alkali land, and can simultaneously degrade phenylacrylic acid, p-hydroxybenzoic acid, ferulic acid and other problems such as salt-tolerant Streptomyces yellow ocher, so as to improve the utilization rate of raw materials, low production cost, The effect of improving stability

Active Publication Date: 2018-11-27
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, although many microorganisms have the ability to degrade phenolic acids, including more than 20 species such as Phanerochaete chrysosporium, Serratia marcescens, Azospirillum adipogenicum, and Bacillus amyloliquefaciens, but isolated from The salt-tolerant Streptomyces ochreus in the cotton rhizosphere soil in saline-alkali land, which can simultaneously degrade phenylacrylic acid, p-hydroxybenzoic acid, ferulic acid and other phenolic acids, has not been reported yet.

Method used

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  • Salt-alkali-resistant streptomyces silaceus and application thereof
  • Salt-alkali-resistant streptomyces silaceus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the isolation of bacterial strain

[0053] The strain was isolated from cotton rhizosphere soil in saline-alkali land. The soil was sampled in Yongan Town, Kenli District, Dongying City, Shandong Province, which was a saline-alkali soil where cotton had been planted continuously for more than 5 years.

[0054] The specific separation method is as follows: weigh 5 g of the mixed soil sample, put it into 100 mL of enrichment medium, and shake and culture at 37 ° C and 150 rpm for 7 days. Take 1 mL of the enriched culture for 10 -1 ~10 -5 Serial dilution, then take 10 -2 、10 -3 、10 -4 The three dilutions were spread on the plate containing the selection medium, and cultured at 37°C for 7 days. Single colonies were picked and streaked onto plates containing selection medium, and cultured at 37°C for 7 days. Pick a single colony and transfer it to the slant of the preservation medium test tube, culture it at 37°C for 7 days, and after it is covered with ...

Embodiment 2

[0061] Embodiment 2: the identification of bacterial strain

[0062] 1. Morphological and physiological and biochemical characteristics:

[0063] The morphological characteristics of the PAPM18 bacterial strain are: a single bacterial colony is flat, round or nearly round on Gao's No. 1 medium, and the surface is dry. The colonies were initially white and gradually turned pale yellow. The central ring is depressed, and there is a concentric ring pattern on the outer edge. The edge is relatively smooth, slightly irregular corrugated, and the back view is earthy yellow. The hyphae in the base have no transverse septa, the aerial filaments are flexible, the spores are straight or flexible, and the surface of the spores is smooth and oval.

[0064] The physiological and biochemical characteristics of the PAPM18 strain are: positive nitrate reduction test, positive starch hydrolysis test, positive D-glucose test, positive D-fructose test, positive sucrose utilization test, positi...

Embodiment 3

[0075] Embodiment 3: Degradation test of Streptomyces yellow ochreus PAPM18 p-phenylacrylic acid, p-hydroxybenzoic acid, ferulic acid

[0076] Prepare the culture medium containing phenylacrylic acid, and its formula is: 1000 mg of phenylacrylic acid, 20 g of soluble starch, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 6g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH=7.4-7.6.

[0077] Prepare a medium containing p-hydroxybenzoic acid, its formula is: p-hydroxybenzoic acid 1000mg, soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 6g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH=7.4-7.6.

[0078] Prepare the culture medium containing ferulic acid, its formula is: ferulic acid 1000mg, soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 6g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH=7.4-7.6.

[0079] Streptomyces chrysogenum PAPM18 was transferred into the above-mentioned three mediums, and cultured with shaking at 37°C a...

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Abstract

The invention discloses streptomyces silaceus PAPM 18 which is already collected at the China General Microbiological Culture Collection Center on July 4, 2018 under a biological collection number ofCGMCC NO. 16054. The strain can survive in saline-alkaline soil, has good degrading effect on plant autotoxins like allyl propionate, p-hydroxybenzoic acid and ferulic acid, can relieve successive cropping obstacle caused by the plant autotoxins and has effects of promoting growth of and increasing yield of cotton cultivated in saline-alkaline land. An active bacterial preparation can be obtainedby performing enlarged culture on the strain, inoculating the strain into a culture medium taking corn starch and corn paste as main nutrition sources, culturing at 35-37 DEG C for 72-96h, adding trehalose, molasses and biological fulvic acid powder into fermentation liquid and spray-drying. A preparation method of the active bacterial preparation is scientific, short in fermentation, high in fermentation liquid bacteria concentration, low in active bacteria loss in the process of spray-drying and high in stability of the active bacteria preparation in the process of preservation.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms and ecological restoration, in particular to a strain of saline-alkali-tolerant Streptomyces ochreus and application thereof. Background technique [0002] The total area of ​​saline-alkali land in my country is as high as more than 500 million mu, and it is of great significance to develop and utilize it reasonably. Commonly used saline-alkali land improvement methods include physical, chemical, water conservancy, biological and other measures, among which biological improvement has the most ecological benefits and development prospects. The application of biological fertilizers is a common method for biological improvement of saline-alkali soil, but whether the functional microorganisms in biological fertilizers can survive in saline-alkali soil directly affects its use effect. Therefore, it is of great significance to screen functional microorganisms that can tolerate salin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/00A01P21/00C05F11/08C12R1/465C09K101/00
CPCA01N63/00C05F11/08C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/465
Inventor 孙中涛刘丽英寇娟妮刘珂欣
Owner SHANDONG AGRICULTURAL UNIVERSITY
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