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Mn2+ ion for promoting proliferation of paenibacillus polymyxa and application method thereof

A polymyx spore-like and application method technology, applied in the fields of biological pesticides and biomedicine, can solve the problems of different effects of promoting proliferation and the like

Inactive Publication Date: 2018-10-09
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Visible, Mn 2+ Different strains have different effects on promoting proliferation, Mn has not been seen 2+ Study on the Proliferation Effect of Ions on Paenibacillus Polymyxa

Method used

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  • Mn2+ ion for promoting proliferation of paenibacillus polymyxa and application method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0006] Embodiment 1 a kind of Fe 2+ Ions promote the proliferation of Paenibacillus polymyxa and its application method

[0007] 1. Materials and methods

[0008] 1.1 Materials

[0009] The bacterial classification used in the present invention is endophytic Paenibacillus polymyxa of ginseng ( Paenibacillus polymyxa ), which was deposited on February 5, 2013 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 7250; Glucose, MnSO 4 ·H 2 O (Beijing Chemical Industry). Purified water was from Hangzhou Wahaha Company; other reagents were of analytical grade.

[0010] 1.2 Instruments and equipment

[0011] CHA-S air bath reciprocating constant temperature shaking incubator; Tecan Infinite® 200 Pro multifunctional microplate reader.

[0012] 1.3 Method

[0013]...

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Abstract

The invention provides Mn2+ ion for promoting the proliferation of paenibacillus polymyxa and the application method thereof. An adopted culture medium is formed by adding Mn2+ into a PDB culture medium till the concentration is 50mg / L, and is equal to MnSO4.H2O with the final concentration of 153.75mg / L. The Mn2+ ion can improve the proliferation biomass of paenibacillus polymyxa, shorten the proliferation lag phase of paenibacillus polymyxa, and ensure that the log phase of proliferation is in advance. The Mn2+ ion has an important theoretical significance on high efficient culture of paenibacillus polymyxa, and has a wide promotion value for the application of relevant biological bacterium agents.

Description

technical field [0001] The present invention relates to a kind of Mn 2+ The ion promotes the proliferation of Paenibacillus polymyxa and its application method, belonging to the technical field of biopesticides and biomedicine. Background technique [0002] Paenibacillus polymyxa in ginseng has a variety of physiological and pharmacological activities. It can produce a series of biologically active substances, such as nitrogenase, plant hormones, polysaccharides, hydrolytic enzymes, antibiotics, etc., which have great application value in biological nitrogen fixation, promotion of plant growth, development of new antibiotics, and inhibition of plant diseases. With the increase of environmental pressure and the improvement of people's health and food safety requirements, microbial pesticides have received more and more attention. The present invention adopts the ginseng endogenous Paenibacillus polymyxa strain isolated from ginseng plants, and the fermentation liquid of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/01
CPCC12N1/20C12N1/38
Inventor 臧埔郜玉钢赵岩祝洪艳何忠梅王立岩肖茹雪张连学
Owner JILIN AGRICULTURAL UNIV
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