Method for extracting agilawood high-quality DNA
A high-quality, agarwood technology, applied in the fields of molecular biology and traditional Chinese medicine identification, can solve the problems of difficulty in extracting high-purity DNA, poor extraction effect, and high cost of agarwood, to improve DNA extraction efficiency, improve quantity and quality, and avoid problems. Extraction of unstable effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] A method for extracting high-quality DNA from agarwood, the steps of which are:
[0044] (1) Take 200 mg of dry Acacia wood, wipe the surface with 75% alcohol to remove surface dirt, dry it, chop it up, put it in a 5 mL centrifuge tube, add liquid nitrogen to fully seal and grind for 5 min.
[0045] (2) Quickly add 1000 μL of preheated DNA extraction solution and shake well; incubate in a 60°C water bath for 8 hours, shaking the centrifuge tube several times during this period; add an equal volume of DNA separation solution, vortex for 30 seconds, and centrifuge at 9000 rpm for 20 minutes at room temperature.
[0046] (3) Transfer the supernatant obtained in step (2) to a new 5mL centrifuge tube to ensure that no residue is sucked in; add 2 times the volume of DNA precipitation solution, mix well by suction, and stand at -20°C for 18h.
[0047] (4) Take out the centrifuge tube from step (3) and cool it at room temperature, add an equal volume of DNA detergent, put it in a...
Embodiment 2
[0069] A method for extracting high-quality DNA from agarwood, the steps of which are:
[0070] (1) Take 200 mg of dried Acacia wood, wipe the surface with bleach and remove the surface dirt, dry it, chop it and place it in a 5 mL centrifuge tube, add liquid nitrogen to fully seal and grind for 5 min.
[0071] (2) Quickly add 2000 μL of preheated DNA extraction solution and shake well; incubate in a 50°C water bath for 3 hours, shaking the centrifuge tube several times during this period; add an equal volume of DNA separation solution, vortex for 30 seconds, and centrifuge at 4500 rpm for 1 minute at room temperature.
[0072] (3) Transfer the supernatant obtained in step (2) to a new 5mL centrifuge tube to ensure that no residue is sucked in; add 2 times the volume of DNA precipitation solution, mix well by suction, and stand at -20°C for 12h.
[0073] (4) Take out the centrifuge tube from step (3) and cool it at room temperature, add an equal volume of DNA detergent, put it ...
Embodiment 3
[0088] A method for extracting high-quality DNA from agarwood, the steps of which are:
[0089] (1) Take 100mg of dry Akiras wood, wipe the surface with 75% alcohol to remove surface dirt, dry and chop, put in a 5mL centrifuge tube, add liquid nitrogen to fully seal and grind for 5min.
[0090] (2) Quickly add 500 μL of preheated DNA extraction solution and shake well. Warm in a water bath at 65°C for 8 hours, during which the centrifuge tubes were shaken several times. Add an equal volume of DNA separation solution, vortex for 30s, and centrifuge at 12000rpm for 1min at room temperature.
[0091] (3) Transfer the supernatant obtained in step (2) to a new 5mL centrifuge tube, making sure not to inhale the residue. Add 2 times the volume of DNA precipitation solution, mix with suction, and let stand at -20°C for 12h.
[0092] (4) Take out the centrifuge tube from step (3) and cool it at room temperature, add an equal volume of DNA detergent, put it in an ice bath for 10 minu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com