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A method for producing glucosamine by fermentation

A technology of glucosamine and fermentation medium, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of low fermentation density of strains, low glucosamine production, affecting the extracellular accumulation of glucosamine, etc. , to achieve the effect of increasing production

Active Publication Date: 2021-03-30
湖南金代科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the process of directly using wild-type Escherichia coli strains for ammonia sugar fermentation production, because glucosamine has a certain stimulating effect on E. The low density leads to low ammonia sugar production, which in turn affects the extracellular accumulation of the target product glucosamine, which has also become a technical problem that limits the process of microbial fermentation to produce ammonia sugar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Specific steps are as follows:

[0056] (1) Put 50mL seed medium in a 250mL shake flask, sterilize at 121°C for 20min, pick an agar block with a diameter of 1cm from the seed slope of Escherichia coli BL21 and inoculate it into the medium, 36°C, 200rpm Cultivate for 10 hours to obtain seed solution;

[0057] (2) Add 2g / L pyruvic acid, 4.5g / L aspartic acid, 0.3g / L inositol in the fermentation medium;

[0058] (3) Put 100mL fermentation medium containing 2g / L pyruvate, 4.5g / L aspartic acid, and 0.3g / L inositol into a 500nL shaker flask, sterilize at 121°C for 20min, and use 10% inoculum The seed solution was inoculated into the medium, and cultured at 36° C. and 280 rpm for 40 hours to obtain a fermentation solution.

[0059] Detect the output of glucosamine in the fermentation broth, and the output is 59g / L.

Embodiment 2

[0061] Specific steps are as follows:

[0062] (1) Put 50mL seed medium in a 250mL shake flask, sterilize at 121°C for 20min, pick an agar block with a diameter of 1cm from the seed slope of Escherichia coli BL21 and inoculate it into the medium, 36°C, 200rpm Cultivate for 10 hours to obtain seed solution;

[0063] (2) Add 2g / L pyruvate, 4.5g / L serine, 0.3g / L inositol to the fermentation medium;

[0064] (3) Put 100mL fermentation medium containing 2g / L pyruvate, 4.5g / L serine, and 0.3g / L inositol in a 500nL shake flask, sterilize at 121°C for 20min, and inoculate the seed solution with 10% inoculum Inoculate into the culture medium, culture at 36° C. and 280 rpm for 40 hours to obtain a fermentation broth.

[0065] Detect the output of glucosamine in the fermentation broth, and the output is 52g / L.

Embodiment 3

[0067] Specific steps are as follows:

[0068] (1) Put 50mL seed medium in a 250mL shake flask, sterilize at 121°C for 20min, pick an agar block with a diameter of 1cm from the seed slope of Escherichia coli BL21 and inoculate it into the medium, 36°C, 200rpm Cultivate for 10 hours to obtain seed solution;

[0069] (2) Add 1g / L pyruvate, 3g / L aspartic acid, 0.2g / L inositol to the fermentation medium;

[0070] (3) Put 100mL fermentation medium containing 1g / L pyruvate, 3g / L aspartic acid, and 0.2g / L inositol in a 500nL shake flask, sterilize at 121°C for 20min, and inoculum with 10% inoculum The seed solution was inoculated into the culture medium, and cultured at 36° C. and 280 rpm for 40 hours to obtain a fermentation broth.

[0071] Detect the output of glucosamine in the fermentation broth, and the output is 46g / L.

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Abstract

The invention discloses a method for producing aminoglucose by fermentation and belongs to the technical field of fermentation engineering. The method disclosed by the invention has the benefits thatthrough inoculating escherichia coli into a fermentation culture medium containing pyruvic acidm, amino acid and / or inositol for fermentation culture, the yield of the aminoglucose produced by the escherichia coli is greatly increased; by utilizing the method disclosed by the invention, the yield of the aminoglucose produced by the escherichia coli can be successfully improved from 22 g / L to 59 g / L.

Description

technical field [0001] The invention relates to a method for producing glucosamine by fermentation, which belongs to the technical field of fermentation engineering. Background technique [0002] Glucosamine (GlcN), also known as ammonia sugar, glucosamine or glucosamine, is a compound in which one hydroxyl group of glucose is replaced by an amino group. It is an important functional monosaccharide and the first amino group whose structure has been confirmed. Monosaccharide. Glucosamine is easily soluble in water and hydrophilic solvents, and its derivative N-acetylglucosamine (GlcNAc) can be obtained by acid hydrolysis. GlcN exists in almost all organisms, including bacteria, yeast, filamentous fungi, plants and animals, and is the main component of glycoproteins and proteoglycans, as well as chitosan and chitin. [0003] Glucosamine is widely used in the fields of medicine, food and health care. For example, because glucosamine can specifically act on articular cartilag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12R1/19
CPCC12P19/26
Inventor 何球山黄海军钟红霞
Owner 湖南金代科技发展有限公司