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Preparation method an application of protein chip for detecting pet infection markers

A technology of protein chips and markers, applied in the biological field, can solve the problems of low cost, low sensitivity, qualitative and non-quantitative, etc., and achieve the effect of reducing detection cost, improving detection efficiency and rapid detection

Inactive Publication Date: 2018-10-09
南京秋浦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are more and more pets, and attention is paid to the health of pets. The importance of CRP, SAA, and CPL indicators in dogs and cats. Various kits on the market are based on Elisa and colloidal gold, with low sensitivity, narrow linear range, and qualitative but not quantitative. The protein chip is based on chemiluminescence, with high sensitivity, wide linear range, quantitative, high joint detection throughput, and low cost

Method used

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  • Preparation method an application of protein chip for detecting pet infection markers
  • Preparation method an application of protein chip for detecting pet infection markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of protein chip that is used for the detection of canine infection marker, the preparation method of described protein chip comprises the steps:

[0027] (1) slide pretreatment;

[0028] ① Soak the glass slide in the glass slide pretreatment solution containing 2% H2SO4 for 20 hours, and then wash it with purified water for 2 to 8 times;

[0029] ② Soak the glass slide in 1% silane aqueous solution for 60 minutes;

[0030] ③Put the soaked glass slides in an oven and bake at 200°C for 1 hour.

[0031] (2) Antibody solution spotting;

[0032] As shown in the table below, the canine CRP monoclonal antibody solution and the canine SAA monoclonal antibody solution were automatically spotted by machine. The concentration of the antibody solution was 0.1mg / mL, and each spot was 20nL. The spot distribution of each antibody was shown in the table below.

[0033] first row

the second list

third column

fourth column

fifth column

HRP antibo...

Embodiment 2

[0039] A kind of protein chip that is used for the detection of dog and cat infection marker, the preparation method of described protein chip comprises the steps:

[0040] (1) slide pretreatment;

[0041] ① Soak the glass slide in the glass slide pretreatment solution containing 2% H2SO4 for 20 hours, and then wash it with purified water for 2 to 8 times;

[0042] ② Soak the glass slide in 1% silane aqueous solution for 60 minutes;

[0043] ③Put the soaked glass slides in an oven and bake at 200°C for 1 hour.

[0044] (2) Antibody solution spotting;

[0045] As shown in the table below, the canine CRP monoclonal antibody solution, canine SAA monoclonal antibody solution and canine CPL monoclonal antibody solution were automatically spotted by machine. The concentration of the antibody solution was 0.1mg / mL. Each spot was 20nL. As shown in the table below.

[0046]

[0047] (3) closed process;

[0048] The prepared slide was submerged in blocking solution (PBS buffer c...

Embodiment 3

[0052] A kind of protein chip that is used for cat infection marker detection, the preparation method of described protein chip comprises the steps:

[0053] (1) slide pretreatment;

[0054] ① Soak the glass slide in the glass slide pretreatment solution containing 2% H2SO4 for 20 hours, and then wash it with purified water for 2 to 8 times;

[0055] ② Soak the glass slide in silane aqueous solution with a mass concentration of 0.5% for 45 minutes;

[0056] ③Put the soaked glass slides into an oven and bake at 180°C for 40 minutes.

[0057] (2) Antibody solution spotting;

[0058] As shown in the table below, the cat CRP monoclonal antibody solution and the cat SAA monoclonal antibody solution were spotted automatically by machine, the concentration of the antibody solution was 0.1mg / mL, and 20nL was spotted for each spot. The spot distribution of each antibody was shown in the table below.

[0059] first row

the second list

third column

fourth column ...

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Abstract

The invention relates to the technical field of biology, in particular to a preparation method an application of a protein chip for detecting pet infection markers. The preparation method of the protein chip comprises the following steps: (1) carrying out pretreatment on a glass slide; (2) performing sample application on an antibody solution; (3) preparing the protein chip by using a sealing process. The protein chip provided by the invention is used for the dog and cat infection markers; after the protein chip technology originated by the company is applied to realization of joint detection,the detection efficiency is effectively improved, and the detection cost is lowered; the inventor develops a protein chip diagnostic kit capable of realizing canine C-reactive protein (CRP) / serum amyloid A (SAA) double-test, canine CRP / SAA / CPL triple-test, feline CRP / SAA double-test and feline CRP / SAA / FPL triple-test; the preparation method has the advantages of being fast, high in efficiency, low in cost, and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of a protein chip for detection of pet infection markers. Background technique [0002] There are more and more pets, and attention is paid to the health of pets. The importance of CRP, SAA, and CPL indicators in dogs and cats. Various kits on the market are based on Elisa and colloidal gold, with low sensitivity, narrow linear range, and qualitative but not quantitative. The protein chip is based on chemiluminescence, with high sensitivity, wide linear range, quantitative, high joint detection throughput, and low cost. Contents of the invention [0003] The purpose of the present invention is to provide a protein chip for the detection of infection markers in dogs and cats, and another purpose of the present invention is to provide a preparation method for the protein chip for detection of infection markers in dogs and cats. [0004] In order ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/54306G01N33/68
Inventor 赵波
Owner 南京秋浦生物科技有限公司
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