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Stingless bee honey extract and extracting method and application thereof

A technology of honey extract, extraction method, applied in the field of biochemistry

Active Publication Date: 2018-10-23
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on the protection of intestinal health of stingless bee honey in my country

Method used

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  • Stingless bee honey extract and extracting method and application thereof
  • Stingless bee honey extract and extracting method and application thereof
  • Stingless bee honey extract and extracting method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] This embodiment provides a stingless bee honey extract and an extraction method thereof. The specific steps are as follows:

[0045] 1) Weigh 50g of stingless bee honey, add 250ml of hydrochloric acid solution (pH 2.0), stir to dissolve, ultrasonicate at 50kHz power for 30min, filter with cotton to form a uniform honey solution;

[0046] 2) Supelco Supelite TM After the DAX-8 macroporous resin is soaked in ethanol for 24 hours, weigh 50g of the activated macroporous resin, wash it twice with water until it is tasteless, pour it into an aqueous solution of stingless bee honey, stir and adsorb for 1 hour;

[0047] 3) Let stand, discard the supernatant, and fill the macroporous resin into the glass column, rinse with 0.1L of pH 2 hydrochloric acid aqueous solution and 0.15L of pure water to remove polar substances such as sugar, and finally Elute with 0.4L absolute ethanol solution and collect the eluate,

[0048] 4) Concentrate under reduced pressure at 40°C to a solid substance...

Embodiment 2

[0051] This embodiment provides a stingless bee honey extract and an extraction method thereof. The specific steps are as follows:

[0052] 1) Weigh 50g of stingless bee honey, add 150ml of hydrochloric acid solution (pH 3.0), stir to dissolve, sonicate at 40kHz power for 60min, filter cotton to form a uniform honey solution;

[0053] 2) Supelco Supelite TM After DAX-8 macroporous resin is soaked in ethanol for 48h, weigh 50g of activated macroporous resin, wash 3 times until it is tasteless, pour it into an aqueous solution of stingless bee honey, stir and adsorb for 2h;

[0054] 3) Let stand, discard the supernatant, and fill the macroporous resin into the glass column. Wash with 0.1L of pH 3 hydrochloric acid aqueous solution and 0.15L of pure water to remove polar substances such as sugar. Elute with 0.4L absolute ethanol solution and collect the eluate,

[0055] 4) Concentrate under reduced pressure at 60°C to a solid substance, redissolve it in 10 mL pure water, and extract wit...

Embodiment 3

[0058] This embodiment provides a stingless bee honey extract and an extraction method thereof. The specific steps are as follows:

[0059] 1) Weigh 50g of stingless bee honey, add 250ml of hydrochloric acid solution (pH 1.0), stir to dissolve, sonicate at 60kHz power for 20min, filter cotton to form a uniform honey solution;

[0060] 2) Supelco Supelite TM After DAX-8 macroporous resin is soaked in ethanol for 12 hours, weigh 50g of the activated macroporous resin, wash it with water once until it is tasteless, pour it into the aqueous solution of stingless bee honey, stir and absorb for 0.5h;

[0061] 3) Let stand, discard the supernatant, and fill the macroporous resin into the glass column. Wash with 0.1L of pH 1 hydrochloric acid aqueous solution and 0.15L of pure water to remove polar substances such as sugar, and finally Elute with 0.4L absolute ethanol solution and collect the eluate,

[0062] 4) Concentrate under reduced pressure at 30°C to a solid substance, re-dissolve in ...

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Abstract

The invention relates to an extracting method of a stingless bee honey extract and application thereof. The extracting method comprises the following steps of (1) dissolving stingless bee honey into awater solution with pH (potential of hydrogen) value of 1 to 3, so as to form a honey solution; (2) adding the activated macroporous resin into the honey solution, and stirring, so as to obtain the macroporous resin adsorbing effective ingredients; (3) filling the obtained macroporous resin in step (2) into an eluting column to spray and elute, and collecting an eluting solution; (4) concentrating the eluting solution at low temperature, dissolving into water again, extracting, and collecting an organic layer, so as to obtain the stingless bee honey extract. The more excellent stingless bee honey extract is provided, and has the advantages that the active ingredient groups can reach good synergistic function; the stingless bee honey extract has more significant application activity on colitis in serendipity, especially ulcerative colitis, and the diseases caused by acute ulcerative colitis induced by glucan sulfate; the broad application prospect is also realized on the treatment of colitis caused by other reasons.

Description

Technical field [0001] The invention relates to the field of biochemistry, in particular to the application of stingless bee honey in the preparation of medicines, foods and health products for treating or preventing colitis. Background technique [0002] The tight junctions between intestinal epithelial cells and intestinal epithelial cells on the lumen side of the intestine constitute the physical barrier function of the intestinal mucosa. The intestinal barrier can effectively prevent harmful substances such as intestinal bacteria and endotoxins from passing through the intestinal mucosa and entering the blood circulation . Among them, tight junctions are extremely important to maintain the integrity and stability of the intestinal mechanical barrier. It consists of three membrane proteins, occludin, claudins, and adhesion molecules, and closed loop proteins (ZO-1, ZO-2, ZO-3). ) And other peripheral protein composition. The destruction and dysfunction of tight junctions wil...

Claims

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Application Information

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IPC IPC(8): A61K35/644A61P1/00A61P1/04A23L21/25A23L33/00
CPCA23L21/25A23L33/00A23V2002/00A61K35/644A61P1/00A61P1/04A23V2200/32
Inventor 王凯吴黎明梁馨文薛晓锋
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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