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A kind of microbial liquid bacterial fertilizer and its preparation method and application

A liquid bacteria and microorganism technology, applied in the field of microbial liquid bacterial fertilizer and its preparation, can solve problems such as single function and poor storage stability, and achieve the effects of increasing yield, improving tea quality, improving soil environment and microbial flora

Active Publication Date: 2020-06-16
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of microbial liquid bacterial fertilizer and its preparation method and application, to solve the problems of existing microbial liquid bacterial fertilizer with single function and poor storage stability

Method used

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  • A kind of microbial liquid bacterial fertilizer and its preparation method and application
  • A kind of microbial liquid bacterial fertilizer and its preparation method and application
  • A kind of microbial liquid bacterial fertilizer and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Obtaining and Identification of Bacillus amyloliquefaciens W208

[0027] 1. Strain isolation

[0028] Soil samples were collected from farmland containing rotten rice straw in Shoushan Village, Shoushan Township, Jin'an District, Fuzhou City, Fujian Province. Weigh 5g of soil sample into a sterilized centrifuge tube, add 5 times the volume of sterile water, filter the soil, take the filtrate and inoculate it on LB plate medium, and cultivate it at a constant temperature at 37°C until colonies appear, then pick it up with an inoculation needle Bacteria that grow well on the edge are inoculated on a new LB plate medium. After the newly inoculated endophytic bacteria grow into colonies, pick the endophytic bacteria on the edge and culture them in streaks. Repeat this process until a single colony is obtained. , and named it strain W208. The LB plate medium includes: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, agar 15g / L, pH value 7.0, and steriliz...

Embodiment 2

[0037] The preparation of embodiment 2 microbial liquid bacterial manure

[0038] (1) Preparation of LB medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, adjust the pH to 7.0, and sterilize at 121°C for 20 minutes.

[0039] Preparation of fermentation medium: use starch wastewater from potato starch factory as raw material, add soybean meal powder 10-30g / L, adjust pH value to 6.0-8.0, and sterilize at 121°C for 20min.

[0040] (2) Inoculate the Bacillus amyloliquefaciens W208 obtained in Example 1 on the LB medium, in a constant temperature shaker at 37°C, vibrate at 180r / min for 24h, and make liquid seeds;

[0041] (3) Inoculate the liquid seed into the fermentation medium in the fermenter at an inoculum size of 5% by mass to volume ratio, aerate, ferment for 72 hours at 37° C., supplement the fermentation medium for the first time at the 24th hour of fermentation, and the volume of liquid replenishment For 50% of the volume of the fermentation broth before s...

Embodiment 3

[0042] Application of Example 3 Microbial Liquid Bacterial Fertilizer in Tea Production

[0043] The test was carried out in Jiaotan Tea Farm (about 1000m above sea level) in Shanghang County, Longyan City, Fujian Province. The tea tree variety was Tieguanyin, and the tree was 12 years old. The planting method is border planting and dense planting, and the planting density is 3000-4000 plants / mu. The physical and chemical index parameters of the initial soil of the tea garden are as follows: pH is 5.8, total nitrogen is 0.203%, total phosphorus is 0.026%, total potassium is 1.12%, alkaline hydrolyzed nitrogen is 138.1mg / kg, available phosphorus is 106.7mg / kg, available potassium is 297mg / kg, Organic matter 45.0g / kg, humic acid 17.1g / kg.

[0044] The test was divided into two groups: blank control group (CK) and test group (W208YF).

[0045] Wherein the blank control group (CK) uses clear water, application rate: 1500L / mu;

[0046] The test group (W208YF) applied the microbi...

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Abstract

The invention discloses a microbial liquid fungus fertilizer. The liquid fungus fertilizer is fermentation liquor of bacillus amyloliquefaciens of which the live bacteria number is 1.8* 108 cfu / ml-2.2* 108 cfu / mL, the bacillus amyloliquefaciens is bacillus amyloliquefaciens W208, and the strain was deposited at China General Microbiological Culture Collection Center under accession number CGMCC No. 13006 in 13 Sep. 2016. The invention further discloses a preparation method of the microbial liquid fungus fertilizer and application thereof in production of tea leaves. The provided liquid fungusfertilizer can remarkably improve a soil environment, and is good in fertility, the yield of the tea leaves can be increased remarkably, the caffeine in the tea leaves is reduced remarkably, the tasteof the tea leaves is improved, the preparation method is simple, the cost is low, the storage stability is good, and the microbial liquid fungus fertilizer is simple to use and environmentally friendly, and plays an important role in promotion of production of tea leaves and sustainable development of an ecological tea garden.

Description

technical field [0001] The invention relates to the technical field of agricultural fertilizers, in particular to a microbial liquid fertilizer and its preparation method and application. Background technique [0002] As a major tea-producing country in the world, China's domestic tea consumption has increased rapidly in recent years, reaching 1.6132 million tons in 2013, an increase of 225% over 2001. During the same period, global tea consumption increased from 2.9858 million tons in 2001 to 4.821 million tons in 2013. The large demand for tea in the market has directly promoted the large-scale planting of tea and the improvement of its quality. At present, China has become the fastest growing tea market with the greatest potential in the world. Improving tea quality and increasing tea output are two key technical issues for enhancing the market competitiveness of tea products and improving the economic benefits of tea production enterprises. Fertilization is one of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C05G3/80C05G5/20C05F17/20A01C21/00C12R1/07
CPCC05F5/002C05G3/00C05G3/80C05G5/20C12N1/20C05F5/004C05F11/08Y02W30/40
Inventor 林斌黄菊青徐庆贤郑怡钱蕾官雪芳张臣心
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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