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A kind of preparation method and application of anthocyanin nano liposome
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A liposome and glycoside nanotechnology, applied in the directions of liposome delivery, application, dairy products, etc., can solve the problems of low encapsulation efficiency and large particle size, and achieve high encapsulation efficiency, simple preparation method, and slow degradation. effect of speed
Active Publication Date: 2022-04-12
ANHUI AGRICULTURAL UNIVERSITY
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[0004] However, when the prior art prepares anthocyanin nanoliposomes, the encapsulation efficiency is low and the particle size is relatively large, all higher than 100nm
Method used
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Embodiment 1
[0029] Preparation of ginhen nano liposomes
[0030] 1.08 g of soy lecithin, 0.18 g of cholesterol, 0.24 g of Tween-80, 0.15 g of blueberry glycoside were dissolved in 100 ml of water, and then 100 ml of pH = 4.0, a concentration of 0.05 mol / L of acetate - sodium acetate The buffer solution is transferred to a rotary evaporator at 40 ° C to evaporate at 40 ° C to form a liposome dispersion, and the liposome dispersion is formed at 4.4 w / cm. 3 Ultrasonic treatment at the power density for 5 min to obtain the glyph nano liposome.
[0031] The preparation rate obtained by the present invention is 91.13%, and the particle diameter is 53.80 nm.
Embodiment 2
[0033] Preparation of ginhen nano liposomes
[0034] 0.8 g of soy lecithin, 0.15 g of cholesterol, 0.20 g Tween-80, 0.06g blueberry chromatysin were dissolved in 80 ml of anhydrous ethanol, and then 80 mL of pH = 4.0, a concentration of 0.05 mol / L, sodium acetate The buffer solution is transferred to a rotary evaporator at 40 ° C to evaporate at 40 ° C for 0.5 h. 3 Ultrasonic treatment under the power density for 3 min to obtain the glycoside nano liposome.
[0035] The preparation rate obtained by the present invention was 89.76%, and the particle diameter was 67.23 nm.
Embodiment 3
[0037] Preparation of ginhen nano liposomes
[0038] 1.2 g of soy lecithin, 0.20g cholesterol, 0.30 g of Tween-80, 0.20g blueberry chloride was dissolved in 120 ml of anhydrous ethanol, and then 120 ml of pH = 4.0, a concentration of 0.05 mol / L of acetate-acetate The buffer solution is transferred to a rotary evaporator at 40 ° C to evaporate at 40 ° C for 0.5 h. 3 Ultrasonic treatment under the power density for 7 minutes to obtain the glyph nano liposome.
[0039] The preparation rate obtained by the present invention was 88.95%, and the particle diameter was 60.27 nm.
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Abstract
The invention discloses a preparation method of anthocyanin nano-liposome, which comprises the following steps: dissolving soybean lecithin, cholesterol, Tween-80 and anthocyanin in absolute ethanol, adding a buffer solution, stirring and reacting, and evaporating The ethanol is removed to form a liposome dispersion, and the liposome dispersion is ultrasonically treated to obtain the anthocyanin nanoliposome. The preparation method of the anthocyanin nano-liposome of the present invention has a simple process, is easy to implement, can effectively stabilize the anthocyanin, inhibit the decomposition and oxidation of the anthocyanin, and has a higher encapsulation efficiency and a smaller particle size than the prior art.
Description
Technical field [0001] The present invention relates to the field of use of patrosin, and in particular, to a method and application of a glycoside nano liposome. Background technique [0002] Leminin is a water-soluble natural edible pigment, which is widely present in nature, and it is a flavonoid polyphenol compound formed in different ways in different ways. Leminosine is often used as a natural food colorant, safe and non-toxic, and has a variety of physiological effects, such as antioxidant, refractive radical function, anti-tumor, anti-inflammatory, antibacterial, anti-cardiovascular disease, blood fat, improve vision, etc. It is known as the seventh large essential nutrient after continuous water, protein, fat, carbohydrate, vitamin, and minerals, and then easily accepted by the people. Despite the huge potential in the food industry, its own stability is easily influenced by the external environment, which is more sensitive to external conditions such as pH, light, tempe...
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