Application of bupropion in preparing drug for treating or preventing influenza virus infection
A technology of bupropion and influenza virus, which is applied in the field of medicine, can solve the problem that bupropion has no anti-influenza virus, and achieve the effects of reducing clinical trial time, good safety, and saving a lot of cost
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Embodiment 1
[0059] Example 1: Evaluation of anti-influenza virus activity of bupropion in U937 cell line
[0060] 1 cell culture
[0061] After two subcultures, the frozen and revived cells were expanded with RPMI-1640 medium containing 10% fetal bovine serum and double antibodies (penicillin 100U / ml, streptomycin 100ug / ml), and the inoculation density was not lower than 5x10 5 cell / ml, passage density not higher than 2x10 6 cell / ml.
[0062] 2 Cytotoxicity detection of bupropion
[0063] U937 cells by 1.5×10 5 Cells / well (volume 100 μl) were inoculated in a 96-well cell culture plate; the drug was prepared with 100 μl of culture medium (RPMI-1640 medium + 10% serum + double antibody) per well, and added to the corresponding cell wells for mixing. Eight concentration gradients were set for the drug, and two replicate wells were set for each gradient concentration. The final concentrations were 6.25 μM, 12.5 μM, 25 μM, 50 μM, 100 μM, 200.0 μM, 400.0 μM and 800 μM. After culturing for...
Embodiment 2
[0076] Example 2: Evaluation of the anti-influenza virus activity of bupropion in A549 cell line
[0077] In this experiment, a method similar to Example 1 was used to evaluate the effect of bupropion in human lung epithelial cells A549.
[0078] Since A549 is an adherent cell, when detecting drug toxicity and antiviral activity, the number of cells required in a 96-well plate is 1.5×10 4 cells / well, and the cells need to be plated 24 hours in advance, and the medium should be changed before infection, and the rest of the operations are consistent with Example 1.
[0079] 1 drug toxicity
[0080] The result is as figure 2 As shown in middle A, after treating A549 cells with bupropion at the highest concentration of 800 μM for 48 hours, there was no significant difference in cell viability compared with the control group, indicating that bupropion had no toxicity to cells at this concentration, and its half toxic concentration CC 50 Greater than 800 μM.
[0081] 2 Antivira...
Embodiment 3
[0085] Example 3: Evaluation of Bupropion's Anti-Other Influenza Virus Activity in U937 Cell Line
[0086] This embodiment uses H3N2 subtype (A / Human / Hubei / 3 / 2005) and H7N8 subtype (A / Duck / Hubei / 216 / 1983) and type B influenza virus (B / Human / Hubei / 1 / 2007) respectively The method of infecting U937 cell line was the same as that in Example 1. The results confirmed that bupropion significantly inhibited the replication of these three influenza viruses in a dose-dependent manner, and belonged to a broad-spectrum anti-influenza virus drug.
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