Full-automatic microfluidic system for sample preparation as well as preparation method and use method of full-automatic microfluidic system

Abstract

The invention discloses a full-automatic microfluidic system for sample preparation as well as a preparation method and a use method of the full-automatic microfluidic system. The system comprises a chip main body and a sample interface module, wherein an inlet, a first chip valve, an amplification chamber, a second chip valve, a product output port and a waste liquid output port which are connected in series are arranged on the chip main body sequentially; the sample interface module is in sealed connection with the chip main body, and comprises a lysate storage pool and an amplification reagent storage pool which are arranged vertically and isolated in a sealed manner, and the lower end of the amplification reagent storage pool is isolated from the inlet in a sealed manner. The system isalso equipped with a unique sample adding module and a liquid storage mode, integrated automatic processing can be performed on a biological sample, an amplification product is produced directly, additional special equipment or instruments for a laboratory such as pipettes, oscillators and centrifuges are not needed at all in the whole process, so that the preparation process can be performed outside the laboratory environment, is simple to operate and can be completed by slightly trained non-professionals, and on-site detection of DNA becomes possible.

Description

technical field [0001] The invention relates to the technical field of biochips, in particular to a fully automatic sample preparation microfluidic system and a preparation method and use method thereof. Background technique [0002] For DNA detection technology, the pre-sample preparation process is particularly important. Sample preparation generally includes the steps of cell lysis, DNA extraction, purification, and DNA amplification. [0003] Existing fully integrated sample preparation systems are generally based on liquid workstation-type high-throughput sample preparation instruments. For the liquid workstation, the principle is to transplant the reagents that originally required manual configuration and transfer to the fixed-program manipulator, and the instrument will automatically complete it. The existing liquid workstation is mainly for DNA extraction, purification and PCR system configuration. Generally, it does not have cell lysis and PCR amplification functi...

AI Technical Summary

Problems solved by technology

For the liquid workstation, the principle is to transplant the reagents that originally required manual configuration and transfer to a fixed-program manipulator to be automatically completed by the instrument. The existing liquid workstation is mainly for DNA extraction, purification and PCR system configuration. Generally, it does not have cell lysis and PCR amplification functions, and the integration level is not high. The preparation process often requires the addition of other special equipment such as centrifuges, vortex shakers, pipettes, PCR thermal cyclers, etc. to complete. Therefore, the entire operation process is very complicated, requiring repeated switching between multiple professional instruments, which is time-consuming and labor-intensive. It often takes several days to collect samples and obtain test results.

Moreover, the whole instrument is very bulky and heavy, and needs to be operated by professionals in a special environment, and it is inconvenient to maintain

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