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Method for rapid propagation and detoxifying of stem tips of fragrant pomelo

A technology of stem tip and pomelo, applied in the field of rapid propagation and detoxification of pomelo stem tip, to avoid the influence of external factors, improve the survival rate, and improve the survival rate

Active Publication Date: 2018-11-06
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the application of this technology in the non-toxic seedling propagation of fragrant pomelo is still a blank

Method used

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  • Method for rapid propagation and detoxifying of stem tips of fragrant pomelo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Primary medium: MS medium + 0.1 mg / L NAA and / or 0.1 mg / L BA, pH 5.6.

[0026] 2) Secondary medium: MS medium + 0.1 mg / L NAA and / or 0.1 mg / L BA + 0.6 mg / L GA, pH 5.6.

[0027] 3) Basic medium: MS medium + 6-BA 0.2mg / L + NAA 0.2mg / L + sucrose 28g / L + agar 6g / L, pH 5.6.

[0028] Taking the configuration of 1L primary medium as an example, the specific preparation process is as follows: weigh the parts of MS medium required for 1L medium to dissolve and mix in sequence; then weigh NAA0.1mg and / or BA0.1mg, respectively added to MS solution to dissolve in turn, constant volume, adjust pH value to 5.6; finally subpackage, sterilize at high temperature 121℃, high pressure 0.1MPa for 15-20 minutes, cool the medium before use.

Embodiment 2

[0030] 1) Primary medium: MS medium + 0.3 mg / L NAA and / or 0.3 mg / L BA, pH 5.8.

[0031] 2) Secondary medium: MS medium + 0.3 mg / L NAA and / or 0.3 mg / L BA + 0.8 mg / L GA, pH 5.8.

[0032] 3) Basic medium: MS medium + 6-BA 0.3mg / L + NAA 0.3mg / L + sucrose 30g / L + agar 7g / L, pH 5.8.

[0033] Taking the configuration of 1L primary medium as an example, the specific preparation process is as follows: Weigh the parts of MS medium required for 1L medium to dissolve and mix in turn; then weigh NAA0.3mg and / or BA0.3mg, respectively added to MS solution to dissolve in turn, constant volume, adjust the pH value to 5.8; finally subpackage, sterilize at high temperature 121℃, high pressure 0.1MPa for 15-20 minutes, cool the culture medium before use.

Embodiment 3

[0035] 1) Primary medium: MS medium + 0.5 mg / L NAA and / or 0.5 mg / L BA, pH 6.0.

[0036] 2) Secondary medium: MS medium + 0.5 mg / L NAA and / or 0.5 mg / L BA + 1.0 mg / L GA, pH 6.0.

[0037] 3) Basic medium: MS medium + 6-BA 0.5mg / L + NAA 0.5mg / L + sucrose 32g / L + agar 8g / L, pH 6.0.

[0038] Taking the configuration of 1L primary medium as an example, the specific preparation process is as follows: Weigh the parts of MS medium required for 1L medium to dissolve and mix in sequence; then weigh NAA0.5mg and / or BA0.5mg, respectively added to MS solution to dissolve in turn, constant volume, adjust pH value to 6.0; finally subpackage, sterilize at high temperature 121℃, high pressure 0.1MPa for 15-20 minutes, cool the medium before use.

[0039] Table 1

[0040]

[0041] It can be seen from Table 1 that the choice of medium components has little effect on tissue culture.

[0042] (2) Rapid propagation and detoxification of grapefruit stem tips

[0043]A method for fast propagati...

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Abstract

The invention relates to the technical field of detoxification and propagation of fruit trees, and in particular relates to a method for rapid propagation and detoxifying of stem tips of fragrant pomelo, and the method comprises the following steps: (1) selection and disinfection of explants, to be more specific, taking a fragrant pomelo tuber in spring or autumn for indoor germination, spraying regularly a bactericide after buds grow up, two weeks later, taking the buds again, after heat treatment in a 5% sodium hypochlorite solution of the buds for 8-10 minutes at 35-40 DEG C, putting the buds on a super clean bench under an anatomical lens for stem tip exfoliation under 8-40 times, and selecting the stem tip or a nucellar tissue of an apical bud for standby use; and (2) primary culture,to be more specific, putting the selected stem tip or the nucellar tissue once again in the 5% sodium hypochlorite solution for treating for 3-5min, and putting the stem tip or the nucellar tissue into a primary medium for rapid propagation and growth. The method for rapid propagation and detoxifying of the stem tips of the fragrant pomelo is established by selection of mediums and hormones in each stage of sterilization and tissue culture of the external explants, provides a theoretical basis and a technical support for factory production of fragrant pomelo tissue culture seedlings, and provides a new way for selection and breeding of new varieties.

Description

technical field [0001] The invention relates to the technical field of detoxification and propagation of fruit trees, in particular to a method for rapid propagation and detoxification of pomelo stem tips. Background technique [0002] Pomelo fruit has high nutritional value and sweet taste. It is a very distinctive local characteristic agricultural product. Planting pomelo is of great significance to increase farmers' income and improve people's living standards. [0003] Huanglongbing is a representative disease of pomelo, which has caused the destruction of millions of pomelo orchards in my country. Virus-free treatment of fine varieties is currently the main measure to prevent and control these diseases. The stem tip microbud grafting detoxification technology is mainly a kind of rapid propagation and detoxification technology developed by utilizing the principle that the plant shoot apex meristem has no virus and the cell division speed is fast. [0004] Since Murashi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张斌袁志辉何福林刘小文刘伟刘春霖
Owner HUNAN UNIV OF SCI & ENG
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