Compound biological soil improver and preparation method thereof

A technology of biological soil improvement and soil improver, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of agricultural product quality decline, groundwater pollution, environmental pollution, etc., and increase crop yield and infection rate. Reduced, significant effect

Inactive Publication Date: 2010-06-30
上海拜森生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to the long-term and large-scale application of simple chemical fertilizers, the pollution of the environment is becoming more and more serious, soil compaction, groundwater pollution, and the decline in the quality of agricultural products have aroused widespread concern.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Prepare LB medium in two 200L fermenters, sterilize at 121°C-125°C for 15-20 minutes, inoculate 10% of Paenibacillus polymyxa and Bacillus subtilis respectively, pass in sterilized air, and culture at 40°C After 20 hours, the number of viable bacteria per milliliter is greater than 1×10 11 Each, each 1Kg of bacteria sludge is centrifugally extracted at a speed of 12000 rev / min on a centrifuge.

[0018] Mix 100g of yeast powder, 100g of seaweed polysaccharide powder, 200g of skimmed milk powder and 20g of sodium chloride to form a protective agent, add appropriate amount of sterile water, add Paenibacillus polymyxa and Bacillus subtilis sludge successively, stir and emulsify at low speed Then pack into a freeze-drying tray, freeze-dry in a vacuum dryer, and obtain Paenibacillus polymyxa and Bacillus subtilis freeze-dried powder 2.2Kg altogether.

[0019] Take 1.0Kg of the above compound biological bacteria as the core, add 300Kg of fermented organic fertilizer from live...

Embodiment 2

[0021] In two 500L fermentors, configure LB medium respectively, sterilize at 121°C-125°C for 15-20 minutes, inoculate 10% Paenibacillus polymyxa and potassium bacteria-jelly-like Bacillus (Bacillus mucilaginosus) respectively, Introduce sterile air, and after 20 hours of incubation at 40°C, the number of viable bacteria per milliliter is greater than 1×10 11 One, each 2Kg of bacteria sludge is centrifugally extracted with the rotating speed of 12000 revs / min on the centrifuge.

[0022] Mix 400g of yeast powder, 300g of seaweed polysaccharide powder, 500g of skimmed milk powder and 30g of sodium chloride to form a protective agent with an appropriate amount of sterile water, and then add Paenibacillus polymyxa sludge and potassium bacteria-jelly-like Bacillus sludge After low-speed stirring and emulsification, put it into a freeze-drying tray, and freeze-dry it in a vacuum dryer to obtain a total of 3.0 Kg of Paenibacillus polymyxa and potassium bacteria-Bacillus jellylike fre...

Embodiment 3

[0025] Obtain Paenibacillus polymyxa and Bacillus subtilis two bacterium freeze-dried powder composite biological bacteria 1.2Kg by the method for embodiment 1.

[0026] Take 0.3Kg of the above-mentioned composite biological bacteria as the core, add 150Kg of livestock and poultry manure fermented organic fertilizer and 150Kg of perlite to mix, and obtain 295Kg of composite biological soil improver after screening, wherein the organic matter content is 21%, and the number of biological viable bacteria is 2.6×10 9 pcs / g.

[0027] Beneficial effects of the present invention are further elaborated below by test example:

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PUM

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Abstract

The invention discloses a compound biological soil improver taking a compound biological bacterium as a core. The invention also discloses a preparation method of the compound biological soil improver, which comprises the following steps of: respectively carrying out liquid deep-layer high-density culture on the soil improver to obtain fermentation broth; carrying gout centrifugal treatment on the fermentation broth and then collecting bacterial mud; adding a protective agent to prepare frozen dry powder; and taking the compound biological bacterium as the core and mixing the required powdery organic fertilizer and perlite to obtain the compound biological soil improver. The compound biological soil improver has high active bacterial quantity, can be preserved at normal temperature for a long term, can be mixed with organic soil and inorganic soil improvers for use without being influenced and has remarkable effect for preventing and controlling the soil-borne disease.

Description

technical field [0001] The invention relates to a soil improver, in particular to a biological compound soil improver; in addition, the invention also relates to a preparation method of the biological compound soil improver. Background technique [0002] my country is a country with a large population and a large agriculture. It uses 7% of the world's land to feed 22% of the world's population. The food issue is always a major issue related to the national economy and people's livelihood. my country has 2 billion mu of arable land and needs 150 million tons of fertilizers annually. It is the world's largest importer of fertilizers and producer of nitrogen fertilizers. Even so, the amount of chemical fertilizers still cannot meet the needs of agricultural development. The reason is that the key problem is that the utilization rate of chemical fertilizers is too low. According to the calculations of the research department, the average utilization rate of nitrogen fertilizer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/00C12N1/20C12N1/14C05F11/08C09K101/00C12R1/01C12R1/065C12R1/885C12R1/12
Inventor 吴瑞兵汪红兵余世贤吴奇能
Owner 上海拜森生物技术有限公司
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