Seedling breeding method of wild bletilla striata

Abstract

The invention discloses a seedling breeding method of wild bletilla striata. The seedling breeding method comprises the following steps: (1) selecting seeds: selecting the seeds in uncracked fruit pods in the embryonic age of 4-6 months, storing the seeds in ventilated, shady and cool places with sunshine, drying the seeds and bagging the dried seeds by using self-sealed bags; (2) disinfecting theseeds: taking bletilla striata fruit pods in step (1), firstly washing the bletilla striata fruit pods with running water and then sterilizing the bletilla striata fruit pods in an aseptic room; (3)culturing the seeds: by cutting the fruit pods and shaking the seeds into a subculture multiplication culture medium for culturing the seeds; (4) carrying out secondary culture: inoculating a seedlinginducing culture medium with budlets cultured in step (3), and culturing for 20-25 days to obtain rooting seedlings; and (5) hardening the seedlings: hardening the rooting seedlings in step (4), transplanting the hardened rooting seedlings into a vessel filled with a substrate and culturing the seedlings. The seedling breeding method is high in seedling rate, low in cost, simple to operate and rapid in breeding speed, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for raising seedlings of wild bletilla striata. Background technique [0002] Bletilla striata, also known as Baiji, Lianjicao, dry root, purple orchid, etc., is a perennial herbaceous bulbous plant of Orchidaceae, and its dried tubers are used as medicine. Modern pharmacological experimental studies have shown that Bletilla striata also has pharmacological effects such as anti-tumor and antibacterial. In addition, Bletilla striata is widely used in landscaping, food processing and industrial raw materials. In recent years, the market demand for bletilla striata raw materials has risen rapidly, and wild bletilla striata has been wildly excavated, resulting in a sharp reduction in its wild resource reserves and is on the verge of extinction. It is listed as one of the wild medicinal plants under key protection by the state. In order to protect the wild resourc...

AI Technical Summary

Problems solved by technology

But also there is following shortcoming: (1) owing to adopting tissue culture, easily pollutes pseudobulb, above-mentioned patented technology adopts toxic washing powder and highly toxic mercuric chloride solution to disinfect pseudobulb, pollutes bletilla striata; (2 ) medium design is unreasonable, resulting in too long seedling time and low seedling rate

But its yield is still not ideal enough, the multiplication effect of the plant needs to be improved, and the phenomenon of leaf yellowing may still occur in the cultivation process