Method for separating nucleotides through ion exchange resin composite chromatography

Abstract

The invention discloses a method for separating nucleotides through ion exchange resin composite chromatography. The method is characterized by comprising the following steps: selecting resins with excellent ion exchange separation effects from multiple kinds of similar anion exchange resins by adopting a batch exchange adsorption rate, wherein the resins are used for chromatographic separation ofcation and anion exchange resin combination; performing gradient elution under neutral or weakly-alkaline conditions (pH of 7-10) by adopting a NaCl aqueous solution, thereby obtaining four single nucleotides. Compared with the prior art, the method disclosed by the invention has an excellent separation effect and high nucleotide adsorption capacity, the resin separation yield can reach 92.5%, acarbon column enrichment method can be saved, crystals can be obtained by direct concentration even without concentrating, the cost is greatly reduced, large-scale industrial production of four high-concentration single nucleotides is easily realized, the cost is low, energy conservation and emission reduction are realized, and the green manufacturing environmental protection concept is met.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a large-scale industrial ion exchange resin combined chromatography separation method for nucleotides. Background technique [0002] In recent years, in special fields such as infant nutrition supplements and genetic engineering, the demand for high-purity natural 5'-nucleotides is increasing. The 5'-nucleotides hydrolyzed by ribonucleic acid (RNA) biological enzymes are Source, no chemically synthesized α isomers and other irreplaceable advantages, more and more people of all ages. In the 1960s, 5'-nucleotides were mainly used as freshness aids, and the separation of RNA enzymatic hydrolysis solution was mainly to obtain purine nucleotides such as 5'-guanylic acid and 5'-adenylic acid, and 5' -Adenylic acid is converted to 5'-inosinic acid. For the separation of the four nucleotides, most of them are laboratory or small-scale production. [0003] Use nuclease P1 or 5'-...

AI Technical Summary

Problems solved by technology

So far, there is no good measurement method for the internal pore size and internal surface area of ​​the resin, and the degree of crosslinking can only represent its approximate range. It is very difficult to make a comprehensive evaluation of the aforementioned various factors that affect the separation effect of the resin.

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