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RNA signal connector, target mRNA translation regulation method, logic gate and application

A signal connector and logic gate technology, applied in the fields of genetic engineering and molecular biology, can solve problems such as increasing the complexity of the system, and achieve the effect of simple structure

Active Publication Date: 2022-01-28
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, like previously reported genetic devices, this system still requires the transfer of exogenous genes encoding large proteins such as dCas9, which further increases the complexity of the system

Method used

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  • RNA signal connector, target mRNA translation regulation method, logic gate and application
  • RNA signal connector, target mRNA translation regulation method, logic gate and application
  • RNA signal connector, target mRNA translation regulation method, logic gate and application

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Embodiment Construction

[0066] The present invention is further described in detail by means of examples below. It should be understood that these descriptions are for the purpose of illustrating the present invention only and are not intended to limit the present invention in any way.

[0067] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. In case of conflict, the definitions in this note shall prevail. The materials, methods, and examples used herein are illustrative only and not intended to be limiting unless otherwise specified.

[0068] I. Materials and methods

[0069] 1. Plasmid construction

[0070] The cDNA sequence of Renilla luciferase / c-Myc / OPN / VEGF / BCL 2 / Bax / p21 mRNA signal connector was designed, and its RNA secondary structure was analyzed with online software. The RNA expressing the antisense region and maintaining the secondary structure of ...

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Abstract

The present application provides an RNA signal connector. The RNA signal connector is an oligonucleotide sequence, including an aptamer domain and an antisense domain, optionally including a nucleic acid switch, the antisense domain is complementary to the target mRNA sequence, and the aptamer domain is used as an input signal ligand binding. The RNA signal connector can be used to regulate the translation of target mRNA in cells. The RNA signal connector can also be used to build biological logic gates, establish the connection between input and output signals, rewire cellular signaling pathways and create feedback loops, silence the expression of survival genes and inhibit the growth of cancer cells, and integrate oncogenic signals It has potential application in transforming into tumor suppressor signal and so on.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to an RNA signal connector, a target mRNA translation regulation method, a logic gate and applications thereof. Background technique [0002] It is the basic function of cells to regulate the expression of related genes after sensing extracellular signals. Cells carry out complex physiological functions, such as cell survival, behavior and recognition, through natural signaling networks. Synthetic biology has developed rapidly in recent years, and one of its important goals is to reshape cell signaling networks at the level of therapeutic and biotechnological applications. Synthetic elements are widely used to construct various novel gene regulation circuits, such as genetic switches, digital logic circuits, artificial signaling pathways and feedback loops. Recently, the inventor has constructed a new class of gene regulation device - "CRISPR signal transd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/11
CPCC12N15/11C12N15/85C12N2840/00
Inventor 刘宇辰陈志聪蔡志明
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN