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Method for separation and purification of cynarin and chlorogenic acid in cynara scolymus by simulated moving bed chromatography

A technology of simulating moving bed, separation and purification, applied in the field of extraction and purification of effective components of natural plants, to achieve the effect of simple treatment process, large treatment capacity and reduction of waste water discharge

Active Publication Date: 2018-11-09
华大国询(厦门)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method described in the current art allows for effective isolating specific compounds called cynylic acids (CA) and chioroanins (CN). These molecules have important applications such as medicine, food science, biotechnology, etc., making them valuable resources that could improve our future economics through reducing costs associated with fossils raw materials. Additionally, these methods allow us to separate other chemical components like flavonoid glycosides and lignans while also minimizing their impact on health issues related to human exposure. Overall, they provide benefits over existing techniques but offer unique features compared to previous technologies.

Problems solved by technology

Technological Problem: Current methods involve laborious processes that require multiple steps or take longer than necessary due to their time required for isolate each component individually. There have been attempts at developing new ways to efficiently separate these compounds without requiring many hours of manual work.

Method used

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  • Method for separation and purification of cynarin and chlorogenic acid in cynara scolymus by simulated moving bed chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Artichoke leaves were dried at 60°C for 12 hours, crushed, passed through a 10-20 mesh sieve, and then refluxed with 5 times the amount (mass volume ratio) of 70% ethanol for 3 times, each time for 3 hours, combined extracts, and reduced pressure After concentrating to a relative density of 1.10, add water for water precipitation, filter to remove insoluble matter, and obtain artichoke leaf extract;

[0040] 2) The artichoke leaf extract obtained in step 1) is processed by macroporous resin column chromatography: select D-101 macroporous adsorption resin, load the sample with a volume flow rate of 2BV / h, use 2BV of 1.0wt%NaOH Aqueous solution to elute impurities, wash with 3.5BV of water to neutrality, elute cynaric acid and chlorogenic acid with 5BV of 75% ethanol, the flow rate of mobile phase is 1BV / h; obtain the crude extract, the volume of ethanol in the crude extract Content > 85%;

[0041] 3) The crude extract is distilled under reduced pressure at 50°C, conc...

Embodiment 2

[0054] 1) Artichoke leaves were dried at 60°C for 12 hours, crushed, passed through a 10-20 mesh sieve, and then refluxed with 10 times the amount of 70% ethanol to extract 3 times, each time for 3 hours, combined extracts, concentrated under reduced pressure to a relative density of After 1.10, add water for water precipitation, filter to remove insoluble matter, and obtain artichoke leaf extract;

[0055] 2) The artichoke leaf extract obtained in step 1) is processed by macroporous resin column chromatography: select D-101 macroporous adsorption resin, load the sample with a volume flow rate of 2BV / h, use 1BV of 1.5wt%NaOH Aqueous solution to elute impurities, wash with 3.5BV of water to neutrality, elute cynaric acid and chlorogenic acid with 5BV of 75% ethanol, the flow rate of mobile phase is 1BV / h; obtain the crude extract, the volume of ethanol in the crude extract Content > 85%;

[0056] 3) The crude extract is distilled under reduced pressure at 50°C, concentrated by...

Embodiment 3

[0069] 1) Artichoke leaves are dried at 60°C for 12 hours, crushed, passed through a 10-20 mesh sieve, and then refluxed with 8 times the amount of 80% ethanol for 2 times, each time for 3 hours. The extracts are combined and concentrated under reduced pressure until the relative density is After 1.10, add water for water precipitation, filter to remove insoluble matter, and obtain artichoke leaf extract;

[0070] 2) The artichoke leaf extract obtained in step 1) is processed by macroporous resin column chromatography: select AB-8 macroporous adsorption resin, load the sample with a volume flow rate of 2BV / h, use 1BV of 1.5wt%NaOH Aqueous solution to elute impurities, wash with 3.5BV of water to neutrality, elute cynaric acid and chlorogenic acid with 5BV of 75% ethanol, the flow rate of mobile phase is 1BV / h; obtain the crude extract, the volume of ethanol in the crude extract Content > 85%;

[0071] 3) The crude extract is distilled under reduced pressure at 50°C, concentra...

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Abstract

The invention discloses a method for separation and purification of cynarin and chlorogenic acid in cynara scolymus by simulated moving bed chromatography (SMBC). SMBC separation technology can achieve continuous feeding, high separation degree and large treatment capacity, can improve the utilization ratio of the filler and mobile phase and reduce the purification cost, and belongs to a green andenvironment-friendly separation and purification process. The method provided by the invention utilizes octadecyl silane bonded silica gel as the stationary phase, and realizes high precision separation of cynarin and chlorogenic acid in cynara scolymus, through standard curve calculation, both the separated products have mass fractions up to 75% or above, and the yields are 60% or more. Separation of cynarin and chlorogenic acid by SMBC not only saves a lot of solvent, but also improves the production efficiency at the same time, and realizes low carbon and environment-friendly economical production. The method provided by the invention combines ethanol reflux extraction technique and macroporous resin column chromatography impurity removal technique, reduces water consumption, lowers energy consumption, has no need for flocculation to remove impurities and special regeneration of the stationary phase, reduces wastewater discharge, and has a simple process.

Description

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Claims

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Application Information

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Owner 华大国询(厦门)生物科技有限公司
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