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Preparation method of yogurt starter freeze-dried powder

A yogurt starter, freeze-dried powder technology, applied in microorganism-based methods, biochemical equipment and methods, milk preparations, etc., to achieve stable structure and flavor, stable acid-producing ability, and good compatibility.

Active Publication Date: 2022-07-01
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest challenge in the mixed fermentation process is the stability of fermentation between batches

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The fermentation preparation method of Lactobacillus bulgaricus and Streptococcus thermophilus mixed bacteria comprises the following steps:

[0029] S1, the preparation of mixed strains: the mixed strains are composed of Lactobacillus bulgaricus and Streptococcus thermophilus, which are stored in the form of glycerol tubes; each glycerol tube contains 3ml of bacterial liquid, and the viable bacteria content per ml of bacterial liquid is: 4.5* 10 9 CFU, the ratio of viable bacteria per milliliter of bacterial solution is: the number of viable bacteria of Streptococcus thermophilus: the number of viable bacteria of Lactobacillus bulgaricus=10:1;

[0030] S2, preparation of Lactobacillus bulgaricus strains: Lactobacillus bulgaricus strains are stored in glycerol tubes; each glycerol tube contains 2ml of bacterial liquid, and the viable bacteria content per ml of bacterial liquid is 4.5*10 8 CFU;

[0031] S3, seed medium: skimmed milk powder: 10%;

[0032] S4, Fermentat...

Embodiment 2

[0045] The fermentation preparation method of Lactobacillus bulgaricus and Streptococcus thermophilus mixed bacteria comprises the following steps:

[0046] S1, preparation of mixed strains: the mixed strains are composed of Lactobacillus bulgaricus and Streptococcus thermophilus, which are stored in the form of glycerol tubes; each glycerol tube contains 3ml of bacterial liquid, and the viable bacteria content per ml of bacterial liquid is: 3* 10 9 CFU, the ratio of viable bacteria per milliliter of bacterial solution is: the number of viable bacteria of Streptococcus thermophilus: the number of viable bacteria of Lactobacillus bulgaricus=10:1;

[0047] S2, preparation of Lactobacillus bulgaricus strains: Lactobacillus bulgaricus strains are stored in the form of glycerol tubes; each glycerol tube contains 2ml of bacterial liquid, and the viable bacteria content per ml of bacterial liquid is 6*10 8 CFU;

[0048] S3, seed medium: skimmed milk powder: 10%;

[0049] S4, Ferme...

Embodiment 3

[0062] The fermentation preparation method of Lactobacillus bulgaricus and Streptococcus thermophilus mixed bacteria comprises the following steps:

[0063] S1, the preparation of mixed strains: the mixed strains are composed of Lactobacillus bulgaricus and Streptococcus thermophilus, which are stored in the form of glycerol tubes; each glycerol tube contains 3ml of bacterial liquid, and the viable bacteria content per ml of bacterial liquid is: 6* 10 9 CFU, the ratio of viable bacteria per milliliter of bacterial solution is: the number of viable bacteria of Streptococcus thermophilus: the number of viable bacteria of Lactobacillus bulgaricus=10:1;

[0064] S2, preparation of Lactobacillus bulgaricus strains: Lactobacillus bulgaricus strains are stored in the form of glycerol tubes; each glycerol tube contains 2ml of bacterial liquid, and the viable bacteria content per ml of bacterial liquid is 3*10 8 CFU;

[0065] S3, seed medium: skimmed milk powder: 10%;

[0066] S4, F...

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PUM

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Abstract

The invention provides a method for preparing yogurt starter freeze-dried powder, which comprises the following steps: preparation of mixed bacterial species: the mixed bacterial species is composed of Lactobacillus bulgaricus and Streptococcus thermophilus, and the ratio of viable bacteria per milliliter of bacterial liquid is: thermophilic bacteria Streptococcus viable count: Lactobacillus bulgaricus viable count = 10:1; Preparation of Lactobacillus bulgaricus strains: Lactobacillus bulgaricus strains were stored in the form of glycerol tubes; Seed medium: skim milk powder: 10%; fermentation culture Base: Maltose: 1%-5%, Glucose 2%-5%, Lactose 2%-10%, Skim Milk Powder 3%-8%, Whey Powder 1%-10%, Magnesium Sulfate 0.1%-1%, Manganese sulfate 0.01%-1%, Tween 80: 0.1%-1%, biotin 0.1%-1%; medium sterilization: sterilize the seed medium, let it cool for use, sterilize the fermentation medium, wait for the temperature Inoculate when cooled to below 37°C. The invention makes full use of the symbiotic relationship between Lactobacillus bulgaricus and Streptococcus thermophilus, and prepares direct-throwing yogurt fermentation bacteria through one-time fermentation.

Description

technical field [0001] The invention belongs to the technical field of yogurt starter, in particular to a preparation method of freeze-dried powder of yogurt starter. Background technique [0002] Direct-cast yogurt fermentation bacteria are the mainstream commercial yogurt fermentation bacteria on the market. The direct-injection yogurt fermentation bacteria is generally composed of several strains such as Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus thermophilus, etc. It is characterized by high content of viable bacteria and strong fermentation activity, and can be directly put into milk or milk powder solution for fermentation Yogurt without traditional transculture. Among them, Lactobacillus bulgaricus and Streptococcus thermophilus are the main fermenting bacteria, but these two bacteria can not generally be used as yogurt fermentation bacteria alone, and there are major defects in the texture and flavor of the final yogurt when used alone, such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123C12R1/225C12R1/46
CPCA23C9/1238C12N1/20A23V2400/123A23V2400/249
Inventor 喻扬
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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