Chemiluminescence immunoassay assay determination kit for detecting human growth hormone and preparation method thereof

A chemiluminescence immunoassay and human growth hormone technology, applied in the field of immunoassay, can solve the problems of poor repeatability, long detection time, low sensitivity, etc.
CN108776232AInactive Publication Date: 2018-11-09DIRUI MEDICAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DIRUI MEDICAL TECH CO LTD
Publication Date
2018-11-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a chemiluminescence immunoassay assay determination kit for detecting human growth hormone and a preparation method thereof, belonging to the technical field of immunoassay. Thekit contains a magnetic bead suspension liquid coated with streptavidin, acridinium ester labeled GH antibody and biotin labeled GH antibody. The invention also provides a preparation method of the chemiluminescence immunoassay assay determination kit for detecting human growth hormone. For the kit, a sandwich method principle is adopted for quantitatively determining GH in serum or plasma, a biotin-GH antibody compound is added to streptavidin-magnetic bead suspension liquid, a magnetic bead-avidin-biotin-GH compound is formed by the affiliation reaction of biotin and streptavidin, and an antigen-antibody compound is formed by antigen-antibody reaction to form magnetic bead-biotin-GH antibody-GH-GH antibody-acridinium ester. The detection method has the advantages of high sensitivity, strong specificity, good repeatability, wide detection scope and relatively low cost, and can be widely applied to clinical detection.
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Description

technical field

[0001] The invention belongs to the technical field of immune analysis, and relates to a chemiluminescence immunoassay assay kit for detecting human growth hormone and a preparation method thereof. Background technique

[0002] Human Growth Hormone is a single peptide chain protein hormone secreted by eosinophilic cells in the anterior pituitary gland. It consists of 191 amino acids and has a wide range of physiological functions. Human Growth Hormone mainly stimulates the liver to secrete insulin-like growth factors. (IGF-I), acts on bone and cartilage, causing height growth, and at the same time can promote human protein synthesis and fat decomposition, and regulate water and salt balance in the body.

[0003] 1. Clinical manifestations of auxin abnormalities

[0004] Growth hormone abnormalities are mainly divided into: 1) growth hormone excess; 2) growth hormone deficiency. Gigantism and acromegaly are commonly seen with growth hormone excess. Gigantis...

Claims

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