Methods for screening nucleic acid aptamers

Abstract

The present invention relates to a method for screening nucleic acid aptamers, which comprises the following steps: (a) contacting target molecules immobilized on a solid phase carrier with nucleic acid aptamer candidates, (b) sorting by capillary electrophoresis The nucleic acid aptamer candidate that has bound to the target molecule, (c) amplifying the nucleic acid aptamer candidate by PCR.

Description

technical field [0001] The present invention relates to a method for screening nucleic acid aptamers. Background technique [0002] The so-called nucleic acid aptamer refers to single-stranded DNA or RNA with molecular recognition ability, which was first reported by Ellington et al. and Tuerk et al. in 1990. Nucleic acid aptamers can be obtained by an evolutionary engineering method called Systematic Evolution of Ligands by Exponential enrichment (SELEX), and a large number of aptamers with binding ability and specificity comparable to antibodies have been reported. Sexual nucleic acid aptamers. In addition, aptamers can be obtained for various targets such as proteins and cells, such as low-molecular compounds that are difficult to obtain antibodies, and their application to therapeutic drugs and diagnostic drugs is expected. However, the acquisition probability of antibodies is said to be 90% or more, whereas the acquisition probability of conventional nucleic acid apta...

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Problems solved by technology

In this method of setting the sorting region, there is a problem that a sequence with low binding ability that dissociates halfway is easily included in the sorting region ( figure 1 A)

[0005] In addition, when small molecules such as low-molecular compounds are used as targets, changes in electrophoretic mobility when binding to ssDNA libraries can hardly be expected, so it is considered difficult to apply CE-SELEX

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