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Multinucleated cell exchange device and method

A technology of multinucleated cells and cells, applied in the field of biological cells, can solve problems such as incomplete removal of cell nuclei, affecting the success rate of nuclear transplantation, cell membrane damage, etc., to achieve the effect of improving work efficiency, improving survival rate, and reducing damage

Inactive Publication Date: 2018-11-13
长沙健金电子技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional cell nuclear transfer methods, such as micromanipulation based on micropipettes and electrofusion transplantation, inevitably cause defects such as damage to large-scale cell membranes, loss of cytoplasm, and incomplete removal of nuclei, which affect the success rate of nuclear transfer.

Method used

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  • Multinucleated cell exchange device and method
  • Multinucleated cell exchange device and method
  • Multinucleated cell exchange device and method

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specific Embodiment

[0049] In actual use, pour cell A and cell B in the culture medium into cell chamber 4; adjust the multiples of electron microscope 1 eyepiece 101 and objective lens 102, so that the electron microscope can clearly see cell A and cell B in cell chamber 4 The cell nucleus structure in; open the optical tweezers emitter 2, make the laser 201 emitted by the optical tweezers emitter 2 form a light beam in the cell chamber 4; move the displacement stage 3, so that the cell A in the cell chamber 4 of the stage 3 moves to the light beam On the light beam formed by the tweezers emitter 2; adjust the intensity of the laser 201 emitted by the optical tweezers emitter 2, so that the light beam breaks the cell wall A1 on the cell A; breaks the cell wall B1 on the cell B in the same way through the above steps; adjust the light The intensity of the laser 201 emitted by the tweezers emitter 2 makes it form optical tweezers in the cell B to clamp the nucleus B2 in the cell B; move the displac...

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Abstract

The invention discloses a multinucleated cell exchange device and method. The multinucleated cell exchange device comprises an optical tweezers cell exchange device, wherein the optical tweezers cellexchange device comprises an electron microscope, an optical tweezers emitter located on the electron microscope, an objective table located below the electron microscope, a cell chamber located on the objective table and an adjusting seat located at the bottom of the objective table; the adjusting seat comprises a hoisting platform and a displacement platform which is arranged on the hoisting platform and is connected with the objective table. The multinucleated cell exchange device disclosed by the invention is simple to operate and convenient to use; cells are subjected to transplanted andexchange by utilizing non-contact, damage-free and high-precision characteristics and the like of optical tweezers, the working effect is easy to improve, damages to the cells are reduced and the survival rate of the cells is improved, so that scientific researches are facilitated.

Description

technical field [0001] The invention relates to the field of biological cells, in particular to a multinuclear cell replacement device and a method thereof. Background technique [0002] Multinucleated cells are protoplasmic masses with more than two nuclei, also known as multinucleated cells or coplasma. The reasons for its formation can be distinguished as: (1) cell fusion, the syncytium produced as a result of cytoplasmic mixing; (2) the cell nucleus has undergone division in monocytes, but the syncytia formed without cell division and its results is different. Examples of (1) can be seen in the laticifers and amoeba-shaped tapetal cells of higher plants, in the muscles and connective tissues of animals; Examples of (2) can be seen in cells, such as the proteus of slime molds and the vegetative body of tube algae. [0003] Cell fusion induced by artificial methods can form two types of binucleated or multinucleated cells. Cells formed by the fusion of cells from the sa...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/36
CPCC12M31/06C12M41/48
Inventor 林建斌
Owner 长沙健金电子技术有限公司
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