Manufacturing method and application of nano diamond and myohemoglobin modification based electrochemical enzyme sensor

A nano-diamond, myoglobin technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve problems such as toxicity hazards and carcinogenicity, and achieve a wide potential window, low background current, and good chemical stability. Effect

Inactive Publication Date: 2018-11-13
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because of its salty taste and low price, it is often used as an unreasonable substitute for table salt in illegal food preparation, but its toxicity is very harmful to human body and it is carcinogenic

Method used

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  • Manufacturing method and application of nano diamond and myohemoglobin modification based electrochemical enzyme sensor
  • Manufacturing method and application of nano diamond and myohemoglobin modification based electrochemical enzyme sensor
  • Manufacturing method and application of nano diamond and myohemoglobin modification based electrochemical enzyme sensor

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Embodiment 1

[0052] The preparation method of Nafion / Mb / ND / CILE comprises the following steps:

[0053] (1) Take graphite powder and ionic liquid (HPPF 6 ), that is, take 1.6 g graphite powder and 0.8 g ionic liquid HPPF 6 Put it in a mortar and grind it fully for 2 hours to get a carbon paste, then fill the carbon paste into a 4 mm glass electrode tube and compact it, and insert a copper wire as a wire to get a carbon ionic liquid electrode;

[0054] Before each electrode modification, the electrode surface needs to be polished to a mirror surface on a polishing paper;

[0055] (2) Take 8 μL of 0.5 mg / mL nano-diamond dispersion drop-coated on the surface of CILE, and let it dry naturally at room temperature to obtain ND / CILE;

[0056] (3) Take 8 μL of 15 mg / mL Mb solution and drop-coat it on the surface of ND / CILE, and let it dry naturally at room temperature to obtain Mb / ND / CILE;

[0057] (4) Take 6 μL of 0.5% Nafion ethanol solution and drop-coat it on the surface of Mb / ND / CILE, and ...

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Abstract

The invention discloses a manufacturing method and application of a nano diamond and myohemoglobin modification based electrochemical enzyme sensor. The manufacturing method comprises the following steps: firstly, weighing and adding a proper amount of graphite powder and ion liquid into a mortar, sufficiently grinding to uniformly mix, filling into a glass electrode tube to compact, internally inserting a copper wire as a guide wire, thereby manufacturing a carbon ion liquid electrode (CILE); dispersing nano diamond (ND) into deionized water to prepare dispersion liquid of different concentrations, performing ultrasonic treatment for 2-3 hours to disperse uniformly, taking and dropwise coating a certain volume of nano diamond dispersion liquid to the surface of CILE, and drying in air atthe room temperature to obtain ND/CILE; fixing myohemoglobin (Mb) to the surface of ND/CILE to dry in air at the room temperature to obtain Mb/ND/CILE; and coating a Nafion alcohol solution to the surface of Mb/ND/CILE, and drying in air at the room temperature to obtain the electrochemical enzyme sensor (Nifion/Mb/ND/CILE). Nifion/Mb/ND/CILE is further taken as a working electrode for being applied to electrochemical detection of targets such as trichloroacetic acid, sodium nitrite and hydrogen peroxide, so that the effective and accurate measurement can be realized, detection response time is short, detection limit is low, linear range is wide, and the stability is good.

Description

technical field [0001] The invention relates to an electrochemical biosensor, in particular to a preparation method and application of an electrochemical enzyme sensor based on nano-diamond and myoglobin modification. Background technique [0002] Electrochemical biosensor is the development product of the combination of electrochemical analysis method and biosensing technology. Due to its strong operability, high sensitivity, fast response and low detection cost, it is widely used in industrial and agricultural production, clinical medicine and environmental monitoring. And many other fields; according to the different biological sensitive elements on the electrode surface, it can be divided into many types, and enzyme sensors (also known as enzyme electrodes) are one of the important branches. The immobilization of biologically active enzymes is a key step in the preparation of enzyme sensors, and choosing a suitable immobilization material is one of the key factors to imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/36
CPCG01N27/308G01N27/3272G01N27/3277G01N27/3278G01N27/36
Inventor 孙伟谢慧牛燕燕牛学良李小宝李晓燕殷春晓翁文举罗贵铃
Owner HAINAN NORMAL UNIV
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