Preparation method of nano biosensor for differentiating different sensitive and drug-resistant cancer cell

Abstract

The invention discloses a preparation method of a nano-biosensor for differentiating sensitive and drug-resistant cancer cells, comprising: the first step, generating hydroxyl groups on the electrode surface: firstly polishing the electrode to the mirror surface, and respectively washing them in acetone, absolute ethanol, ultrapure Wash it in water, dry it, put it in phosphate buffer solution (PBS) with a pH of 5.0, and treat it with an electrochemical multi-potential step method. After the treatment, it will be scanned continuously by cyclic voltammetry. Washing and drying; the second step, nanoparticle modification: mix the aqueous solution of functionalized metal nanoparticles or polymer nanofibers at a volume ratio of 1:1 and pH=7.2, 0.1mol / l HEPES buffer, and then add 0.5 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and mix evenly. Finally, soak the electrode pretreated in the first step in the modification solution, and it will be ready after 10 hours. Get nano biosensors. Sensitive and drug-resistant cancer cells can be distinguished by electrochemical methods or by measuring the contact angle of different cancer cells on the surface of the sensor by using the nano-biosensor.

Description

technical field [0001] The invention relates to a biosensor based on a nano-interface, in particular to a preparation method of a nano-biosensor for differentiating sensitive and drug-resistant cancer cells. Background technique [0002] Cancer has become one of the biggest killers that endanger human health today, and how to effectively diagnose and treat it has always been a difficult problem that the medical field has been working hard to overcome. After more than half a century of development and improvement, surgery, radiotherapy, chemotherapy and other methods have become the main means of comprehensive treatment of malignant tumors, but they often cause serious side effects in clinical application, and the therapeutic effect is not very good. A large number of clinical facts have proved that the cure rate of early cancer is quite high. Therefore, the key to treating cancer lies in effective early diagnosis and treatment. At present, the most effective means of diagn...

AI Technical Summary

Problems solved by technology

SELDI protein chip technology (also known as SELDI-TOF-MS technology) has greatly accelerated the screening and research of tumor markers, and has very important clinical application value in the early diagnosis of tumors, but there are also some shortcomings, such as operation The process is cumbersome and complicated, the capture ability of the chip is limited, the difference between chip batches and the low resolution of mass spectrometry make the reproducibility of the research results poor, and the obtained peptide peaks are few.

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