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Camphor free whole genome SSR molecular marker, preparation method and application thereof

A genome-wide, molecular marker technology, applied in biochemical equipment and methods, recombinant DNA technology, DNA/RNA fragments, etc., can solve the lack of camphor tree EST data, it is difficult to meet the needs of camphor tree EST-SSR marker development, and limit camphor tree molecules. Development of biological research and other issues

Active Publication Date: 2018-11-16
JIANGXI ACAD OF FORESTRY
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  • Abstract
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  • Claims
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Problems solved by technology

However, compared with other species such as rice, corn, grapes, etc., the EST data of camphor trees included in the database of the National Center for Biotechnology Information (NCBI) is quite lacking, which is difficult to meet the needs of camphor tree EST-SSR marker development, and cannot be used for the development of camphor tree EST-SSR markers. DNA molecular marker development research provides sufficient information
This also greatly limits the development of camphor tree molecular biology research

Method used

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  • Camphor free whole genome SSR molecular marker, preparation method and application thereof
  • Camphor free whole genome SSR molecular marker, preparation method and application thereof
  • Camphor free whole genome SSR molecular marker, preparation method and application thereof

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Embodiment 1

[0077] 1. The preparation method of primers for SSR molecular markers developed based on the genome of camphor tree, comprising the following steps:

[0078] 1. Camphor tree DNA extraction

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Abstract

The invention discloses a camphor free whole genome SSR molecular marker, a preparation method and application thereof. A camphor tree microsatellite molecular marker can be applied to population genetic diversity detection and genetic relationship analysis of ancient camphor trees, provides a bath of novel microsatellite molecular markers for lauraceae, and further provides a novel tool for researches on population genetic differentiation and structure and population genetic diversity level, gene flow and mating system among populations, speciational evolution and marker assisted selection oflauraceae.

Description

Technical field: [0001] The invention belongs to the field of forestry molecular biotechnology, in particular to a camphor tree molecular marker technology, and more particularly to a camphor tree whole genome SSR molecular marker and a preparation method thereof, as well as its application in the genetic diversity analysis and kinship identification of ancient camphor. Background technique: [0002] Microsatellites are also known as Short Tandem Repeats (STRs), Simple Sequence Repeats (SSRs), and Simple Sequence Length Polymorphisms (SSLP). It refers to a DNA sequence of 1 to 6 nucleotides repeated in tandem for many times, which is commonly found in the genomes of eukaryotes and prokaryotes, and even exists in virus genomes. SSR molecular markers have the following characteristics: highly variable repeat units and repeat times, high polymorphism information capacity; co-dominant, inherited according to Mendelian laws; relatively conservative flanking sequences; neutral sel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6806C12N15/11
CPCC12Q1/6806C12Q1/6895C12Q2600/156C12Q2531/113C12Q2525/151
Inventor 伍艳芳徐海宁王建汪信东郑永杰刘新亮戴小英邱凤英杨海宽
Owner JIANGXI ACAD OF FORESTRY
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