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Method of detecting content of amino acids in volvaria volvacea
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An amino acid and straw mushroom technology, applied in the field of amino acid detection, can solve the problems of slow growth rate, low density and low accuracy, and achieve the effects of fast growth rate, high density and thick mycelium.
Inactive Publication Date: 2018-11-16
SHANGHAI ACAD OF AGRI SCI
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In addition, the accuracy of existing methods for detecting amino acid content in straw mushrooms is low and cannot reflect the actual content of amino acids in straw mushrooms
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Embodiment 1
[0095] The straw mushroom medium provided in this example contains 200 parts by weight of potatoes, 20 parts of glucose, 5 parts of peptone, 10 parts of agar, and 25 parts of soybean meal water extract.
[0097] Boil 25g of soybean meal with 500mL of water for 20 minutes, filter, and take the filtrate to obtain the aqueous extract solution of soybean meal, about 300mL, and set aside.
[0098]Taking the preparation of 1000ml straw mushroom culture medium as an example, the preparation method of straw mushroom culture medium is described as follows:
[0099] Weigh 200g of potatoes, cut into small pieces about 3cm long, add 500ml of water, boil for 15-20min; then use gauze to filter, take the filtrate, which is the potato water extract solution. Take 300ml of potato water extract solution, add 20g of glucose, 5g of peptone, 25g of soybean meal water extract solution and 10g of aga...
Embodiment 2
[0102] The straw mushroom culture medium that present embodiment provides, by weight, it contains 200 parts of potatoes, 20 parts of glucose, 5 parts of peptone, 10 parts of agar, 25 parts of soybean meal water extract, and mass volume ratio (g:ml) is 0.5% calciumsulfate.
[0103] Wherein, taking the straw mushroom culture medium of preparing 1000ml as an example, the preparation method of the straw mushroom culture medium is described as follows:
[0104] Get potato water extract solution (its preparation method is the same as Example 1) 300ml, 20g glucose, 5g peptone, 25g soybean meal water extract, appropriate amount of calciumsulfate dihydrate and 10g agar join in the filtrate, add water to make up to 1000mL, pH Naturally, autoclave, 121°C, 20min. Wherein, the added amount of calciumsulfate dihydrate can ensure that the final concentration of calcium sulfate in the 1000ml straw mushroom culture medium is 0.5%.
Embodiment 3
[0106] The composition and preparation method of the straw mushroom culture medium provided in this example are the same as those in Example 2, except that the concentration of calcium sulfate is 1%.
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Abstract
The invention discloses a method of detecting content of amino acids in volvaria volvacea and relates to the technical field of amino acid detection. The method includes the steps of: adding hydrochloric acid into a hydrolysis tube filled with a volvaria volvacea sample, performing vortex vibration for first extraction; adding hydrochloric acid and performing vortex vibration for secondary extraction; supplying the hydrochloric acid to preset volume, volume-to-mass ratio of the preset volume to the volvaria volvacea sample being 10-15:1. During measurement of the content of amino acids, the hydrochloric acid is stepwisely added to the sample for hydrolysis, so that the amino acids in the sample are fully dissolved out, and difference between detected amino acid content and actual amino acid content is reduced; therefore, reliability of a detection result is improved.
Description
technical field [0001] The invention relates to the technical field of amino acid detection, in particular to a method for detecting amino acid content in straw mushrooms. Background technique [0002] Strawmushroom (Volvariella volvacea (Bull.: Fr.) Sing.), also known as orchid mushroom and baojiao mushroom, originated in Nanhua Temple in Shaoguan, Guangdong. Artificial cultivation began in my country 300 years ago, and it was introduced by overseas Chinese in the 1930s. All over the world, it is an important tropical and subtropical mushroom and the third largest cultivated edible fungus in the world. The output of straw mushroom in my country ranks first in the world, and it is mainly distributed in South China. [0003] Straw mushroom has high nutritional and edible value because of its unique flavor, high nutritional content and good medicinal effect. [0004] Straw mushroom is a kind of high-quality edible fungus. The level of protein content is usually used as an im...
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